鸡ApoA5基因的多态性与胆固醇浓度和繁殖性状的关联分析.pdfVIP

中国农业科学 2013,46(17):3704-3711 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.17.020 鸡ApoA5 基因的多态性与胆固醇浓度和繁殖性状的关联分析 杜晓惠, 熊 婷, 刘俊莹, 张怀勇, 万火福, 朱 庆 （四川农业大学动物科技学院，四川雅安625014 ） 摘要： 【目的】探究ApoA5 基因的多态性与胆固醇浓度和繁殖性状之间的关系，为平衡胆固醇浓度与繁殖性 状间的关系奠定基础。 【方法】试验共记录了434 只SD 母鸡的繁殖性能、300 d 时的血清胆固醇浓度（SCC）和 蛋黄胆固醇浓度（YCC），采用PCR-SSCP 及PCR-RFLP 技术，筛查到ApoA5 基因的6 个新的SNPs 并进行关联分析。 【结果】单个SNP 的效应比较集中在开产日龄（AFE）和SCC 上；由位置靠近、效应一致的SNP2、SNP4 和SNP5 构 建的单倍型，关联分析结果大致与单个SNP 相吻合，但更突显了各性状在单倍型组合间的差异，SCC 和YCC 都较 高的H5H8、H4H8、H5H6、H6H6 单倍型组合具有较好的繁殖性能，SCC 和YCC 都较低的H1H5、H1H6 和H3H8 组合则 相反。 【结论】ApoA5 基因的单倍型SNP2-4-5 能够揭示各组合在胆固醇及各繁殖性状间的差异，可以作为提高繁 殖性能的分子标记。 关键词：鸡; ApoA5 基因; 多态性; 胆固醇; 繁殖性状; 关联分析 Polymorphisms of Chicken ApoA5 Gene and Their Associations with Cholesterol Content and Reproductive Traits DU Xiao-hui, XIONG Ting, LIU Jun-ying, ZHANG Huai-yong, WAN Huo-fu, ZHU Qing (College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, Sichuan) Abstract: 【Objective 】To balance the relationship between cholesterol concentration and reproductive traits, the associations among the traits of cholesterol and reproduction and polymorphisms of chicken ApoA 5 gene were studied. 【Method 】 The reproduction traits were recorded and the cholesterol concentrations of serum (SCC) and egg yolk (YCC) were measured in 434 SD hens at 300th day. Through PCR -SSCP and PCR-RFLP analyses, 6 new SNPs were detected in ApoA5 gene and their associations with the traits of cholesterol and reproduction were analyzed. 【Result 】The effects of the single SNP mainly focused on the age of first egg (AFE) and on the SCC. The results of association analysis of the haplotype, constructed with SNP2, SNP4 and S