鸡FATP1基因多态性与屠体性状的相关分析.pdfVIP

中国农业科学 2009,42(9):3272-3278 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.09.033 鸡FATP1基因多态性与屠体性状的相关分析 张 龙，朱方俊，王 彦，兰 茜，吕荣平，刘益平，朱 庆 （四川农业大学动物科技学院/ 四川农业大学国家级动物类实验教学示范中心，四川雅安 625014 ） 摘要：【目的】研究FATP1基因多态性与优质肉鸡屠体性状的相关性，为进一步加快育种进程提供参考依据。 【方法】利用PCR-SSCP技术对381只大恒优质鸡FATP1 基因的部分外显子进行群体遗传学检测，计算基因频率和 基因型频率，对多态性与屠体性状进行相关分析。【结果】在FATP1 P3 和P4两个位点上分别存在3 种多态以及9 种单倍型组合。P3位点不同基因型对活重、屠体重、半净膛、全净膛、胸肌重和腿肌重有显著影响，P4位点不同 基因型对腿肌重和腹脂率有显著影响。9种单倍型组合AAMM、AAMN、AGMM、AGMN、AANN、AGNN、GGMM、GGMN 和GGNN 的频率分别为0.538、0.052、0.265、0.029、0.045、0.037、0.024、0.005和0.005，不同单倍型与腹脂重、胸 肌重和胸肌率存在相关性。【结论】鸡FATP1不同基因型和单倍型组合与部分屠体性状和腹脂率存在相关性。 关键词：鸡；FATP1基因；多态性；单倍型；PCR-SSCP Correlation Analysis Between FATP1 Gene Polymorphism and Carcass Traits in Chicken ZHANG Long, ZHU Fang-Jun, WANG Yan, LAN Xi, LÜ Rong-Ping, LIU Yi-Ping, ZHU Qing (Animal Science and Technological College/Na tional Experimental Teaching Demonstration Center of Animal Sciences, Sichuan Agricultural University, Ya’an 625014, Sichuan) Abstract: 【Objective 】 The polymorphisms of FATP1 and their correlation to carcass traits in chicken were investigated for purpose of providing molecular maker information to facilitate the breeding efficiency. 【Method 】 PCR-SSCP was applied to analyze the polymorphisms of partial exon loci in FATP1 gene in 381 Chinese chicken from Dahen breeding company. Genotype frequencies, allelic frequencies, correlation analysis between the polymorphisms and carcass traits were estimated. 【Result 】 The result showed that 3 genotypes and 9 haplotypes combinations were found at the two loci. The genotypes of P3 loci had a significant effect on live weight, carcass weight, semi-eviscerated weight, eviscerated