小麦抗叶锈病基因Lr19的AFLP标记.pdfVIP

中国农业科学 2005,38(9):1796-1800 Scientia Agricultura Sinica 小麦抗叶锈病基因 Lr19 的AFLP标记 李 星，杨文香，李亚宁，刘大群，闫红飞，孟庆芳，张 汀 （河北农业大学植物病理系，河北省农作物病虫害生物防治工程技术研究中心，保定 071001 ） 摘要：以Thatcher和23个以Thatcher为遗传背景的小麦抗叶锈病近等基因系及TcLr19与Thatcher杂交 F2代植株为材料，利用AFLP技术开展了小麦抗叶锈病基因Lr19 的分子标记研究。共获得7个与小麦抗叶锈病基 因连锁的分子标记：P-AGT/M-GAG （3.3cM）、P-ACA/M-GGT （4.1cM）、P-ACA/M-GGT （4.1cM）、P-AAC/M-CAG 289bp 102bp 106bp 123bp （4.9cM）、P-AAC/M-GGT （5.0cM）、P-ACA/M-GGT （5.7cM）和P-ATC/M-GAG （9.6cM）。这些特异性片段经 203bp 290bp 293bp 回收、克隆、测序得出了特异带的序列。该研究可促进遗传图谱、物理图谱的构建和小麦抗叶锈病基因Lr19的克 隆。 关键词：小麦；Lr19；分子标记；AFLP Identification of AFLP Markers Linked to Lr19 Resistance to Wheat Leaf Rust LI Xing ，YANG Wen-xiang ，LI Ya-ning ，LIU Da-qun ，YAN Hong-fei ，MENG Qing-fang ，ZHANG Ting (Department of Plant Pathology ，Agricultural University of Hebei ，Biological Control Center of Plant Diseases and Plant Pests of Hebei Province ，Baoding 071001) Abstract ：AFLP analysis was carried out on Thatcher, twenty three near­isogenic lines and F generation of Tclr19 ×Thatcher 2 and developed molecular markers for gene Lr19 resistance to wheat leaf rust. Seven markers linked to Lr19 resistance traits were obtained, which were P-AGT/M-GAG289bp (3.3cM), P-ACA/M-GGT102bp (4.1cM), P-ACA/M-GGT106bp (4.1cM), P-AAC/M- CAG123bp (4.9cM), P-AAC/M-GGT203bp (5.0cM), P-ACA/M-GGT290bp (5.7cM), and P-ATC/M-GAG293bp (9.6cM). All of these specific fragments were isolated from the polyacrylamide gels, re-amplified, cloned and sequenced. The research may faci