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phKL基因和Ph2基因缺失重组体的创制及其与小麦外源物种杂种的染色体减数分裂观察.pdf
维普资讯
植 物 学 报
ActaBotanicaSinica2003,45(9):1121—1128 http://www.chineseplantscience.com
CombinationofHomoeologousPairingGenephKLandPh2-deficiencyin
CommonW heatandItsM eioticBehaviorsinHybridswithAlienSpecies
LIUDengCai*,ZHENGYou—Liang,YANZe—Hong,ZHOUYong—Hong,V~%IYu—M ing,LANXiu—Jin
(TriticeaeResearchInstitute,SichuanAgriculturalUniversity,DujiangyanCity,Sichuan611830,China)
Abstract: ThecombinedlineshavingbothphKLandPh2-deficiencywereobtainedinthegeneticback—
groundofcommonwheat(TridcumaestivumL.)landrace.Theselineshadnormalfertility.Inthewheat
combinedlines×Aegi/opsvariabilisEig.(orrye),asignificantincreaseinthechiasmataofhomoeologous
pairingwasshownbythephKL+Ph2一plantswithrespecttotheirphKL+Ph2sibswhichindicatesthatPh2-
deficiencyandOhKLshowedanadditiveeffectonpromotingpairing.The effectswereshowninthe
incrementofrodbivalents.ringbivalentsandtrivalentsandreductionofunivalents,ofwhich,reductionof
univalentswasmainlyduetotheincrementofrodbivalents.Thecombinedlinesareprobablymoredesir—
ablematerialsforaliengenetransferringthanphKLorPh2一linesalone.Incomparisonwiththatof0h1b
×Ae.variahs(orrye).phKL+PhZ~×Ae.vadabi//s(orrye)showhigher(orsimilar)numbersofrod
bivalents,whilethetotalchromosomepairinglevelsignificantlyreducedthatascribedtothedecrementin
ringbivalentsandmultivalentsTheseresultsprobablyindicatethedifferentgeneticmechanismsforPh1
andPh2orphKLoncontrollinghomoeologouspairing.
Keywords: Tr/ticumaestJvum :homoeologouspairing;Phgene;metaphase—Ipairing;interspecific
hybrid
Commonwheat(Triticum aestivum L.,2n=42, srtengthofphlinwheat—alien,thoughhavingnodetect—
AABBDD)is0.1qallohexaploidspecieswithgenomesA,B ableeffec
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