脂多糖刺激对断奶仔猪脾脏、胸腺和外周血白细胞PPARγ蛋白表达的影响.pdfVIP

中国农业科学 2008,41(7):2149-2153 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2008.07.037 脂多糖刺激对断奶仔猪脾脏、胸腺和外周血 白细胞PPARγ蛋白表达的影响 鲁 晶，刘玉兰，石君霞，侯永清，刘洪明，朱惠玲，丁斌鹰，郭广伦 （武汉工业学院饲料科学系/动物营养与饲料科学湖北省重点实验室，武汉 430023 ） 摘要：【目的】研究免疫应激对断奶仔猪脾脏、胸腺和外周血白细胞过氧化物酶体增殖物活化受体γ（PPARγ） 蛋白表达水平的影响。【方法】选取 12 头健康断奶仔猪，分成 2 组，每组 6 个重复。试验组注射 100 μg·kg-1 BW 的脂多糖（LPS）建立免疫应激模型，对照组注射等量生理盐水。注射后3 h，采血分离血浆和白细胞待测，然后 立即屠宰仔猪，取脾脏和胸腺，通过Western blot 测定脾脏、胸腺和白细胞中PPARγ蛋白表达水平。【结果】LPS 刺激仔猪，导致血浆白细胞介素-6﹑肿瘤坏死因子-α、皮质醇和前列腺素E2 含量显著升高（P ＜0.05），胰岛素、 类胰岛素生长因子-1 含量显著降低（P ＜0.05）；LPS 刺激导致血浆葡萄糖、总蛋白、白蛋白和球蛋白含量显著降 低（P ＜0.05）；LPS刺激导致脾脏（P ＜0.05）、胸腺（P ＜0.1）和白细胞（P ＜0.05）PPARγ蛋白表达水平升高。 【结论】脂多糖剌激可提高仔猪脾脏、胸腺和外周血白细胞PPARγ蛋白的表达，显示PPARγ可能参与仔猪免疫应 激的调控，可能成为缓解仔猪免疫应激的一个新靶点。 关键词：断奶仔猪；脂多糖；PPARγ Effect of Lipopolysaccharide Challenge on PPARγ Protein Expression in Spleens, Thymus and Peripheral Leucocytes of Weaned Pigs LU Jing, LIU Yu-lan, SHI Jun-xia, HOU Yong-qing, LIU Hong-ming, ZHU Hui-ling, DING Bin-ying, GUO Guang-lun (Hubei Key Laboratory of Animal Nutrition and Feed Science/Department of Feed Science, Wuhan Polytechnic University, Wuhan 430023) Abstract: 【Objective 】 An experiment was conducted to investigate the effect of immunological stress on peroxisome proliferator-activated receptor γ (PPARγ) protein expression in spleens, thymus and peripheral leucocytes of weaned pigs. 【Method 】 Pigs were allocated to two treatment groups (6 replicates/treatment and 1 pig/replicate). The pigs in treatment group were injected with 100 µg·kg-1 BW lipopolysaccharide (LPS), whereas in control group were injected with an equivalent amount of sterile sal