中国小麦条锈菌生理小种演化及遗传重组.pdfVIP

中国农业科学 2011,44(9): 1815-1822 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578 -1752.2011.09.007 中国小麦条锈菌生理小种演化及遗传重组 詹刚明，王建锋，王晓杰，黄丽丽，康振生 （西北农林科技大学植物保护学院/ 陕西省农业分子生物学重点实验室，陕西杨凌 712100 ） 摘要： 【目的】探索中国小麦条锈菌主要生理小种毒性演化关系及小种间的亲缘关系，以期寻找生理小种遗 传重组的分子证据，进而揭示中国小麦条锈菌生理小种毒性变异机制。【方法】采用鉴别寄主分析毒性法和 TP-M13-SSR 荧光标记技术，对12 年来收集的21 个菌系进行毒性鉴定，并对其夏孢子基因组DNA 进行SSR 标记分 析。【结果】毒性分析和SSR 分析各将供试菌系聚为4 个类群，两者的相关系数为r=0.21。另外，13 对SSR 引物 中有2 对引物显示条锈菌生理小种存在重组现象。【结论】中国条锈菌生理小种毒性多样性丰富，但遗传分化较 低，条锈菌夏孢子阶段的体细胞遗传重组可能为毒性变异的主要方式之一。 关键词：小麦条锈菌；毒性分析；SSR 标记；遗传重组 Evolution and Genetic Recombination of Physiological Races of Puccinia striiformis f. sp. tritici in China ZHAN Gang-ming, WANG Jian-feng, WANG Xiao-jie, HUANG Li-li, KANG Zhen-sheng (College of Plant Protection, Northwest Agriculture and Forestry University/Key Laboratory of Molecular Biology for Agriculture of Shaanxi, Yangling 712100, Shaanxi) Abstract: 【Objective 】 The objective of this study is to explore the virulence evolution and relative relationship in main physiological races of Puccinia striiformis f. sp. tritici (Pst) in China, for searching the molecular evidence of somatic genetic recombination and revealing the virulence variation mechanism of physiological races of Pst in China. 【Method 】Virulence of 21 races collected during the past 12 years were tested on Chinese differentials, and then the genomic DNA isolated from them were used for SSR analysis by TP-M13-SSR technique. 【Result 】Twenty-one races were clustered into 4 groups in both virulent analysis and SSR markers, the correlation index between them is 0.21. Furthermore, 2 pairs of primers among 13 pairs showed the existence of recombinant among the