中国抗枯、黄萎病陆地棉材料分子水平的遗传差异评价.pdfVIP

中国农业科学 2005,38(5):936-943 Scientia Agricultura Sinica 中国抗枯、黄萎病陆地棉材料分子水平的遗传差异评价 温小杰，马峙英，王省芬，张桂寅，李喜焕 （河北农业大学／河北省作物种质资源重点实验室，保定 071001 ） 摘要：采用SSR和AFLP分子标记技术，对中国不同年代培育的54个抗枯、黄萎病陆地棉品种（系）分子水 平的遗传差异进行了研究。22对SSR引物和20个AFLP 引物组合共扩增出446个多态性标记，每个品种平均8.26 个。采用SPSS11.5软件计算欧氏距离，中国抗病品种间欧氏距离分布在5.568～11.790之间，平均值为8.906； 单一品种欧氏距离平均值为8.530～10.011，高于欧氏距离总平均值的品种共10个，占品种总数的18.52%；85.55% 的品种欧氏距离值分布于 7.000～10.000 之间，表明中国供试抗病品种群体的遗传基础较为狭窄。作为比较对照 的4个国外品种间欧氏距离变化在9.055～10.724之间。国内品种与国外品种之间的欧氏距离介于6.481～12.490， 82.02%的品种欧氏距离值分布于 8.000～11.000 之间，表明国外品种与国内品种之间遗传差异较大。基于 SSR 和 AFLP整合数据的聚类分析，58个品种被划分为5个SAG（SSRAFLP-based groups）分子类群，SAG的划分与品种 系谱来源有较强的相关性。 关键词：棉花；抗枯、黄萎病；遗传差异；SSR；AFLP Molecular Divergence Estimation of Chinese Upland Cottons with Fusarium and Verticillium Wilt Resistance WEN Xiao-jie, MA Zhi-ying, WANG Xing-fen, ZHANG Gui-yin, LI Xi-huan （Key Laboratory for Crop Germplasm Resources / Hebei Agricultural University, Baoding 071001 ） Abstract: SSR(simple sequence repeats) and AFLP(amplified fragment length polymorphism) were used to assess the genetic divergence of 54 Chinese cottons with Verticillium and Fusarium wilt resistance. 446 DNA polymorphic markers were scored from amplifications of 22 SSR primers and 20 AFLP primer pairs, with 8.26 markers for each variety in average. Euclidean distances were calculated using the software of SPSS version 11.5 and dendrograms were constructed. The Euclidean distances were 5.568-11.790, with the mean Euclidean distance 8.906. Average genetic distances for every single variety were 8.530-10.011, and those of 10 varieties, accounted for 18.52% of the total, were above the total mean Euclidean distance. The genetic distances of 85.55% of the varieties ranged from 7.000 to 10.000. All of above re