乌司他丁对兔髓细胞中inos及mmp-2、3表达的影响.pdfVIP

目 录 一、摘要 中文论著摘要·····················································1 英文论著摘要················································．．．．．3 二、英文缩略词·······················································．5 三、论文 —1，·．L 日U吾。。。。。。‘。。。‘。。‘。。’。。。‘。·······································6 材料与方法················································．．．．．．．7 实验结果·······················································．10 讨论········”··················································13 结论····························································14 四、本研究创新性的自我评价···········································15 五、参考文献························································．16 六、附录 综述····························································18 在学期间科研成绩··············································．·27 致谢··········································-·················28 个人简介························································29 ·中文论著摘要· 乌司他丁对兔髓核细胞中iNOS及MMP-2、3 表达的影响 目 的 本课题以兔椎间盘髓核细胞为研究对象，并用IL．1p对髓核细胞进行诱导。后 测定髓核细胞中iNOS及MMP．2、3的表达，探讨不同浓度的乌司他丁对白介素一 1B诱导的兔腰椎间盘髓核细胞中iNOS及删P一2、3表达的影响，为乌司他丁运用于 临床缓解椎间盘退变以及退变所导致的疼痛等提供了一定思路。 ‘ 方 法 用酶消化法提取兔椎间盘髓核细胞并进行体外培养；用HE染色法及II型胶原 免疫细胞化学染色法对兔椎间盘髓核细胞进行鉴定；将兔髓核细胞随机分为6组： 正常对照组、乌司他丁对照组(加入320U／ml乌司他丁)、白介素1p诱导组(加入 IL一1 10ng／ml 13)和3个不同浓度乌司他丁抑制组(加入IL-113的同时，分别加入 细胞中MMP．2、3表达变化，并用分光光度法检测各组培养液中iNOS活性。 结 果 HE染色及II型胶原免疫细胞化学染色法表明所提取细胞为正常兔椎间盘髓核 细胞。细胞免疫组化检测提示正常对照组对iNOS及MMP．2、3的表达呈弱阳性， 乌司他丁对照组与正常组相比表达有下降：诱导组iNOS及MMP．2、3的表达呈强 阳性；各抑制组MMP一2、3表达均低于诱导组(P0．01)，且其表达降低的程度与 药物的处理浓度呈正比；各抑制组iNOS含量均低于诱导组，以320U／ml抑制组最显 著(P0．01)。 结 论 在体外条件下，一定浓度的乌司他丁能够抑制IL-1B诱发的椎间盘细胞中 MMP一2、3及iNOS的表达及其含量的增加，减少对蛋白多糖及胶原的降解。 关键词 髓核：胰蛋白酶抑制剂：基质金属蛋白酶一2、3：白细胞介素一1