依达拉奉对大鼠缺血再灌注损伤的影响.pdf

录 ·····························000···············1 ”…····…······一·…···”…···············3 血再灌注损伤的影响 ···”···-··············”····…·…···…··…5 ·················000···························5 ……···…·····…·······00 e···”··········13 ·············································l5 附表············0OOO00·····…··········…···…·····…·················…··…21 讨论…········…·…···························……····…····…··…········22 结论················…··············…························……………···25 OOO O 参考文献……………………………………………J 0……………26 综述 氧自由基与肾缺血再灌注损伤……………………………………29 致谢·········……···……………······……·…···…········…···…···…37 个人简历……………………………………………………………………38 摘要 血再灌注损伤的影响 要 目的：观察不同剂量依达拉奉预处理和后处理联合对大鼠肾缺血再灌 注损伤的影响，并探讨其机制。 氯醛3ml／kg麻醉后，制备肾脏缺血再灌注损伤模型。开腹后，用无损伤 动脉夹同时夹闭双侧肾蒂，开始计时，肉眼观察肾脏颜色变为暗红色即可 确认肾脏血流阻断，造成双侧肾缺血，缺血模型成功。双侧肾缺血45min 后，松开动脉夹，肾脏由暗红色恢复鲜红色即可确认血流恢复，夹闭和开 放肾蒂时腹腔内保留林格液20ml／kg。S组仅行开腹，游离出双侧肾脏， 分离双侧肾蒂不夹闭，伤口用生理盐水纱布覆盖，暴露45min不作肾缺血 处理；I组夹闭双侧肾蒂，缺血45min后松开动脉夹；El组缺血前和再灌 注即刻经尾静脉注入依达拉奉总量lmg／kg；E3组缺血前和再灌注即刻经 尾静脉注入依达拉奉总量3mg／kg；E5组缺血前和再灌注即刻经尾静脉注 入依达拉奉总量5mg／kg。再灌注6h，麻醉后迅速经心脏取血和切取肾标 本，待测。酶法测定血清肌酐(Cr)水平、苦味酸不除蛋白法测定尿素氮 量、黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活性；采用免疫组化(SP) 法观察肾组织中凋亡蛋白Bcl．2和Bax的表达，计算蛋白阳性染色指数 (positive 组织中凋亡细胞，计算凋亡指数(甜)；苏木精．伊红(HE)染色，在光镜 下观察肾组织病理学变化。 结果-与S组比较，缺血再灌注6h时I组、E1组、E3组、 E5组 的BUN、 Cr升