甲壳质衍生物对鼠骨折愈合的作用机制研究.pdf

摘 要 目的：复制大鼠胫骨中段骨折模型，探究三种甲壳质衍生物(N．乙酰氨基葡萄 糖、壳寡糖、壮骨液)对大鼠骨折愈合的作用和机制。方法：手术制备大鼠胫骨中 段骨折模型，实验设计为甲壳质衍生物处理组(N．乙酰葡萄糖组、壳寡糖组、壮骨 液)和模型组，于骨折术后7d、14d、21d，X线摄片和Alcian blue／HE学染色观察骨 CellNuclear 亡率；酶生化法检测血液中钙、、磷离子浓度和碱性磷酸酶(Alcoline phospharase． 壮骨液组骨痂生长愈合早于N．乙酰氨基葡萄糖组和模型组，骨痂中小梁骨占骨痂总 为新形成的软骨细胞和骨痂中的成骨细胞凋亡最高峰；各时阳J段壳寡糖和壮骨液组 性(PO．01 鼠胫骨骨折愈合，其机制与促进骨折部位骨细胞增殖、抑制细胞凋亡、提高血钙磷 细胞ALP、OsteocalcinmRNA表达促进细胞早中期分化。 关键词：甲壳质衍生物；大鼠骨折；成骨细胞；碱性磷酸酶；骨钙素 Abstract effects Toestablishrattibialfracturemodeland the and 0BJ-ECTIVE investigated kindof chitin and mechanismsofthree different tibialfracture solution theratfracture Establishrat Zhuanggu)on healing．METHODS intofour derivativetreatment andtheratsweredivided groups：chitin and controlmodel and solution 4day oligarsaccharideZhuanggu)and group．7day，1 afterthefracture and andAlcian 21 treatment，the days operation X·rayradiography blue／HE were toobservethe offracture stainingperformed histologicalchanges healing， and cellnuclear wereused proliferating staining fordectectthethe of of theterminal abilityproliferationosteocytes．And