丹参对家兔同种体骨移植愈合的影响.pdfVIP

目 录 中文摘要 ···············································1 英文摘要 ···············································2 引 言 ················································4 正 文 ················································5 1.实验材料 ·············································5 2.试验方法 ·············································5 3.结果 ··················································7 4.讨论 ·················································10 结 论 ···············································19 参考文献 ··············································20 附图 ···················································25 综 述 ···············································32 致 谢 ···············································45 攻读学位期间发表的学术论文目录 ····················46 作者声明 ··············································47 个人简历 ··············································48 中 文 摘 要 目 的：本课题通过观察丹参注射液对家兔同种异体骨骨移植愈 合的影响，为临床上应用丹参促进同种异体骨移植愈合提供理论基 础。 方 法：用成年家兔55只，其中7只取其四肢骨用来制备深低温冷冻 同种异体骨段。余48只在3%戊巴比妥钠耳缘静脉注射全麻下，手术制 作左侧股骨中段截除约1-1.5cm长骨干及骨膜（部分截断约股骨周径 l/3，保留对侧皮质骨），植入深低温保存的同种异体皮质骨段，建立 动物模型。术后随机将其分为两组，分笼喂养，自由活动及进食，实 验组除每日给予注射丹参注射液外，其他饲养条件完全与对照组相 同。分别于术后2周、4周、8周、12周处死动物，取骨移植段标本行 大体标本、HE组织学观察，以及骨形态发生蛋白(bone morphogenetic protein, BMP)免疫组化染色观察。 结 果： 1、同种异体骨段标本HE染色显示丹参实验组较之对照 组在术后各时间段软骨细胞及破骨细胞形成，软骨内成骨和膜内成 骨、骨痂形成及改建以及髓腔再通等方面均更早, 实验组骨痂数量及 密度大于对照组； 2、BMP免疫组织化学染色显示实验丹参组同种异体骨移植局部 BMP的阳性及强阳性表达均早于对照组。 结 论：丹参可促进血管的再生、间充质细胞的募集与分化、成 骨细胞及软骨细胞的增值和分化，促进破骨细胞的形成、异体骨的吸 收、骨痂的形成与改建、髓腔的再通，最终加速同种异体骨骨移植愈 合。 关键词：丹参；同种异体骨移植；骨形态蛋白 1 Analyze the influence of rabbits ′BMG healing treated with salvia ABSTRACT Objective : To oberserve the mechanism of allogeneic transplant bone healing with salvia miltiorrhiza injection in the