六味地黄丸（颗）对哮喘小鼠糖皮质激素受体mRNA水平调节作用的研究.pdfVIP

目 录 中文摘要 ···················································1 英文摘要 ···················································3 引 言 ····················································5 正 文 ····················································7 1.实验材料和方法 ·········································7 1.1 实验对象·················································7 1.2 动物饲养条件 ·············································7 1.3 主要试剂和药品 ···········································7 1.4 实验器材·················································7 1.5 实验方法·················································8 1.6 数据统计处理 ············································11 2.实验结果 ···············································11 3.讨 论 ················································14 4.存在的问题和展望 ······································29 结 论 ··················································31 参考文献 ··················································32 综 述 ··················································37 致 谢 ··················································46 攻读学位期间发表的学术论文目录 ·······················47 作者声明 ··················································48 个人简历 ··················································49 中 文 摘 要 目 的：六味地黄丸 （颗粒）是传统中医滋补肾阴的经典代表名方，在 临床上用于防治哮喘取得了满意的疗效，但其作用机理尚待研究。本研 究旨在通过测定哮喘小鼠肺组织内糖皮质激素受体（glucocorticoid receptor ，GR ）ｍRNA 水平，从基因水平探讨该方对糖皮质激素受体的 调节作用，为更好的发挥中医药防治哮喘的优势作用及临床用药提供理 论基础和实验依据。 实验方法：将 100 只健康Balb/C 雄性小鼠随机分为5 组（每组20 只）， 即正常对照组、哮喘模型组、六味地黄组、六味地黄+地塞米松组、地塞 米松组。第0、7、14 天除正常对照组外，给小鼠腹腔注射0.2ml 的致敏 原/生理盐水悬液致敏；于实验第7 天即第二次致敏的同一天开始，每天 上午8:00 予六味地黄组、六味地黄+地塞米松组小鼠六味地黄颗粒按生药 20g ／kg/天灌胃，其余各组均以等量生理盐水灌胃；第 15 ～21 天每天喂 药后除正常对照组外分组将致敏的小鼠臵于密闭容器内用超声雾化器雾 化吸入2%OVA 生理盐水悬液，每天30 分钟，进行激发，正常对照组以 等量生理盐水雾化，同时六味地黄+地塞米松组、地塞米松组分别于每次 激发前1 ｈ腹腔注射10mg/kg 地塞米松，其余各组以等量生理盐水腹腔注 射。各组除施加以上药物因素处理外其日常饲养方法均相同，可以自由 进食常规饲料和水。在末次激发后24 h 内处死小鼠，去肺组织提取RNA ， 反转录合成第一链 cDNA，运用荧光定量PCR 法对 GRmRNA