野生狗牙根种质遗传多样性的SRAP研究.pdfVIP

HEREDITAS (Beijing) 2008 1 , 30(1): 94―100 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2008.00094 SRAP 易杨杰, 张新全, 黄琳凯, 凌瑶, 马啸, 刘伟 , 625014 摘要：采用SRAP 分子标记技术, 对采自中国四川、重庆、贵州、西藏四省区的32 份野生狗牙根(Cynodon dactylon) 材料进行遗传多样性分析, 获得下述结果：(1)用 14 对引物组合共得到 132 条多态性条带, 平均每对引物扩增 出9.4 条多态带, 多态性位点百分率为79.8%, 材料间的遗传相似系数范围在 0.591 到0.957 之间, 平均 GS 值 为 0.759, 这些结果说明, 供试野生狗牙根具有较为丰富的遗传多样性; (2)对所有材料进行聚类分析, 可聚为4 类, 大部分来自相同或相似生态地理环境的材料聚为一类, 表明供试材料的聚类和其生态地理环境间有一定的 相关性; (3)基于 Shannon 多样性指数估算了6 个狗牙根生态地理类群内和类群间的遗传分化, 发现类群内遗传 变异占总变异的65.56%, 而类群间遗传变异占总变异的34.44%; (4)对各生态地理类群基于Nei 氏无偏估计的遗 传一致度的聚类分析表明, 各生态地理类群间的遗传分化与其所处的生态地理环境具有一定的相关性。 ：狗牙根；SRAP；遗传多样性；聚类分析 Genetic diversity of wild Cynodon dactylon germplasm detected by SRAP markers YI Yang-Jie, ZHANG Xin-Quan, HUANG Lin-Kai, LING Yao, MA Xiao, LIU Wei Department of Grassland , Sichuan Agricultural University, Ya’an 625014, China Abstract: Sequence-related amplified polymorphism (SRAP) molecular markers were used to detect the genetic diversity of 32 wild accessions of Cynodon dactylon collected from Sichuan, Chongqing, Guizhou and Tibet,China. The following results were obtained. (1) Fourteen primer pairs produced 132 polymorphic bands, averaged 9.4 bands per primer pair. The percentage of polymorphic bands in average was 79.8%.The Nei’s genetic similarity coefficient of the tested accessions ranged from 0.591 to 0.957, and the average Nei’s coefficient was 0.759. These results suggested that there was rich genetic diversity among the wild resources of Cynodon dactylon tested. (2) Thirty two wild accessions were cl