双向电泳原理和流程.pptVIP

Title or job number Proteomics Proteomics 一个细胞在特定生理或病理状态下表达的所有种类的蛋白质称为蛋白质组(proteome) 仅仅从基因组学水平上无法彻底的了解各种生命现象, 因为蛋白质才是生命活动的真正执行者 从基因组学上我们无法检测到转录后修饰、翻译调节、选择性剪切、以及蛋白复合物形成、蛋白质相互作用等生命现象; 此外并不是细胞内的所有的DNA都翻译转录成蛋白质 Proteome Analysis and Proteomics The analysis of the entire PROTEin complement expressed by a genOME, or by a cell or tissue type. Wasinger VC et al, Electrophoresis 16 (1995) “Proteomics is the study of quantitative changes of expression levels and their application to drug discovery, diagnostics and therapy.” Two basic technologies: 2-D electrophoresis of complex protein mixture