机械制图平时作业课程.docVIP

《机械制图》平时作业 作图题(每题25分,共100分) 一 求直线AB的真实长度,并求直线与V面的倾角α? 解:作图过程如图红色线所示: 做X1//a’b’ 按照投影变换的基本作图法分别作出点A,B的H1面投影a1,b1,连线a1,b1,即为AB的真长?a1,b1与X1的夹角就是AB对V面的倾角α? 二 求作直线AB和直线CD的公垂线EF? 解:利用直角投影定理解题AB垂直V, EF垂直AB 所以EF//V,并且垂足E的投影e’一定积聚在a’b’上 因为EF// V, EF垂直CD 所以e’f’垂直c’d’ 因为EF//V,则e’f’反映EF的真长? e’f’,ef即为公垂线的两面投影? 三 判断点K和直线MS是否在平面MNT上? 解:若点位于平面内的任一直线,则点在该平面内若一直线通过平面内的两点,则该直线在该平面内如图,点K不在?MNT平面上 直线MS不在?MNT平面上 四 作出平面四边形ABCD上的三角形EFG的V面投影? 解:如图? 三角形e’f;g;是EGF在V面上的投影? Acknowledgments The authors would like to thank Johns Hopkins University for the TC-1 cells. This work was supported by a National Health Research Institutes intramural grant (IV-103-PP-22) and grants from the National Science Council, which were awarded to Y.C. Song (NSC 99-2321-B-400-004-MY3) and S.J. Liu (NSC 103-2321-B-400-008). Author Contributions Y.C.S. and S.J.L. designed the studies. Y.C.S. performed the research and analyzed the data. Y.C.S. and S.J.L. wrote the manuscript. Additional Information C57BL/6 mice were immunized subcutaneously (s.c.) once with 1 μ g of peptide mixed with or without 10 μ g of CpG adjuvant. After one week, splenocytes were harvested, and the response of IFN-γ -secreting cells was determined by ELISPOT after 48 h of peptide stimulation. Briefly, 2 × 105 splenocytes were incubated with 1 μ g/ml irrelevant peptide or RAH peptide in an anti-IFN-γ -coated polyvinylidene fluoride (PVDF) plate for 48 h. After incubation, the cells were removed, and a biotinylated anti-IFN-γ Ab (eBioscience, San Diego, CA, USA) was added to each well. The plates were incubated at 37 °C for 2 h. Following the addition of the avidin-HRP reagent (eBioscience, CA, USA), the assay was developed using a 3-amine-9-ethyl carbazole (AEC; Sigma-Aldrich, MO, USA) staining solution. The reaction was stopped after 4–6 min by placing the plate under tap water. The spots were counted using an ELISPOT reader (Cellular Technology Ltd., Shaker Heights, OH, USA). For RAH-specific T cell staining, splee