三株芸豆叶际拮抗细菌的筛选及鉴定.pdfVIP

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 2010 8 1 1 1 1 1 1, 2 路晓萌 姚良同 宋洪宁 丁延芹 侯启会 杜秉海 1 2 ( , 271018; , 271018) : 以芸豆锈病病原菌[ Uromy ces app end iculatus( Pers. )Ung. ]为指示菌,从芸豆叶际 筛选到 3株具有明显拮抗效 果的细菌, 编号为 SS2, L14b, NEW2经过形态学观察, 生理生化测定, 16S rDNA 序列及系统发育分析, 初步鉴定 SS2为短短 芽孢杆菌 (B rev ibac illus brev is ), L14b 与 NEW 2为枯草芽孢杆菌(B ac illus subt ilis) SS2, L14b, NEW 2的 16S rDNA 序列的 en Bank登录号分别为 EU771078EU771076和 EU771079 : 芸豆锈病 拮抗菌 叶际 筛选 鉴定 Screening and Identification of Three Antagonistic Bacteria from Kidney Bean Phyllosphere 1 1 1 1 1 1, 2 Lu X iaomeng Yao Liangtong SongHongning Ding Yanqin Hou Q ihui Du Binghai 1 2 ( Colleg e of Lfi e S ciences, S hand ong Ag ricultura l Un iv ersity, T a ian 271018; K ey La bora tory of Ag ricultura lM icrob io log y, S hand ong Ag ricultura l Un iv ersity, T a ian 271018) A bstrac:t W ith Uromyces app end icula tus ( Pers. ) Ung. as indicator, three antagonistic bacteriawere screened from the phyllo sphere ofK idney bean and named as SS2, L14b, NEW2, respectively. Based on the morphologic characteristics, physiological and bio chem icalproperties and phylogenetic analysis of 16S rDNA, SS2was prelmi inary identified asB rev ibacillus brev is, andL14b and NEW 2 were prelmi inary identified asB acillus subtilis. The sequences of 16S rDNA obtained in this study have been subm itted to enBank and the accession numbers are SS2 asEU771078, L14b as EU771076, andNEW2 as EU771079, respectively. K ey w ords: Uromy