Hegazy’ Simplified Method of Tissue Processing (Consuming Less Time and Chemicals).pdfVIP

Original Article Hegazy’ Simplified Method of Tissue Processing (Consuming Time and Chemicals) 1 2 Raafat Hegazy , Abdelmonem Hegazy 1Department of Pathology, Faculty of Medicine, Zagazig University, Zagazig, Egypt. 2Department of Anatomy Embryology, Faculty of Medicine, Zagazig University, Zagazig, Egypt. ABSTRACT Background: Processing is the next step in the histological process after tissue fixation. There are three methods commonly used for such tissue processing. They are the routine manual, rapid manual and the microwave methods. This study aimed to proceed a simple new manual method in a trial to take the advantages of rapid manual and microwave methods and avoid their disadvantages. Methods: One hundred samples of different tissues and cell blocks were included in this study. They were divided into two equal halves. One half is processed by the routine manual method and the other managed by new suggested technique. Results: The time consuming in the new method was about 7 hours vs. 20 hours in the routine processing. Also, the histologic quality was better in the new method as compared to the routine manual technique. Conclusions: The current simplified method of tissue and cell block processing using mild temperature and moderate agitation possess the advantages of reduction of time of processing, as well as the economic benefit of the utilization of fewer fluids. Key Words: Tissue processing, Cell blocks, Manual method, Histological process INTRODUCTION Microwave method is a recent technique, in which