肝细胞生长因子和复发性阿弗他溃疡关系研析.pdfVIP

山西医科大学硕士学位论文 肝细胞生长因子与复发性阿弗他溃疡关系的研究 摘 要 目的：通过了解复发性阿弗他溃疡 (Recurrent aphthous ulcer ，RAU) 患者溃疡期和间 歇期唾液中肝细胞生长因子 (Hepatocyte growth factor ，HGF) 质量浓度的差异，初步探讨 HGF 在RAU 发生及愈合中的作用。 方法：选择符合纳入标准的30 例轻型RAU 患者，分别在其溃疡期和间歇期(溃疡愈 合后一周内)采集唾液，同期选择 30 例健康成人采集其唾液，采用酶联免疫吸附法 (enzyme-linked immunosorbent assay，ELISA) 检测三组唾液样本中HGF 的质量浓度。统 计学分析，采用单因素方差分析 (one-way ANOVA) 进行三组比较及LSD-t 检验进行两两 比较。 结果：酶联免疫吸附法检测三组唾液样本得出的HGF 质量浓度经统计学分析得到结 果如下： (1) 溃疡期组、间歇期组和对照组三组唾液样本中HGF 质量浓度的差异有统计学意 义(F=12.63 ，P0.01) ； (2) RAU 患者溃疡期唾液中HGF 质量浓度低于间歇期和对照组(LSD-t 检验P0.0 1) ； (3) RAU 患者间歇期唾液中HGF 质量浓度高于对照组 (LSD-t 检验P0.05) 。 结论：初步说明RAU 患者口腔溃疡的发生可能与唾液中HGF 质量浓度降低有关， 而其自愈可能与溃疡产生后唾液中HGF 质量浓度升高有关。 关键词：肝细胞生长因子；复发性阿弗他溃疡；唾液 I 山西医科大学硕士学位论文 Study of relationship between recurrent aphthous ulcer and hepatocyte growth factor in saliva Abstract Objective: To investigate relationship between recurrent aphthous ulcer (RAU) and hepatocyte growth factor (HGF) in saliva by examining the concentration changes of HGF in saliva of RAU patients during the ulcerous and interval period. Methods: HGF levels in saliva samples from 30 RAU patients not only during ulcerous period but also interval period and 30 normal persons were measured by enzyme-linked immunosorbent assay (ELISA). All the 30 patients were minor aphthous ulcers and their ulcers occurred less than four days and the interval period was restricted in a week after ulcer healing. One-way analysis of variance(one-way ANOVA) was used for comparison of three saliva groups, least significant difference t (LSD-t)