红芪有效部位对肺间质纤维化模型大鼠肺组织胶原面积、.docVIP

红芪有效部位对肺间质纤维化模型大鼠肺组织胶原面积、 　　摘要：目的 观察红芪对肺间质纤维化模型大鼠肺组织胶原面积、透明质酸（HA）及层黏连蛋白（LN）表达的影响，探讨其作用机制，并筛选红芪最佳有效部位。方法 将144只SPF级Wistar大鼠随机分为空白组、模型组、强的松组，以及红芪多糖、黄酮、皂苷的高、中、低剂量组。采用气管滴注博莱霉素法建立肺纤维化模型，从造模后第2日起，各药物组分别给予相应剂量药物，每日1次，共28 d。Masson染色观察肺组织胶原纤维的变化，放射免疫法检测各组大鼠肺组织匀浆中HA、LN含量。结果 与模型组比较，红芪黄酮各剂量组、红芪多糖低剂量组肺泡炎明显减轻。红芪黄酮低剂量组、红芪多糖中剂量组、红芪皂苷低剂量组肺组织胶原面积明显减小。与模型组比较，红芪多糖高剂量组、红芪黄酮各剂量组、红芪皂苷高剂量组HA含量明显下降。红芪多糖高剂量组，红芪黄酮中、低剂量组，红芪皂苷中、低剂量组LN含量明显下降。结论 红芪多糖、黄酮、皂苷在适宜剂量下均能减轻肺泡炎症，抑制胶原纤维增生、沉积，降低肺组织中HA、LN含量，其中红芪黄酮效果较佳。 中国论文网 /6/view-7254760.htm 　　关键词：肺纤维化；红芪有效部位；胶原面积；透明质酸；层黏连蛋白；大鼠 　　DOI：10.3969/j.issn.1005-5304.2016.04.019 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）04-0072-05 　　Effects of Active Ingredients in Radix Hedysari on Collagen Area， Hyaluronic Acid and Laminin of Lung Tissues in Rats with Pulmonary Interstitial Fibrosis SU Yun， ZHANG Yi， LI Juan， LI Xue-yan， LIU Yong-qi， LIN Xing-yao， LI Jin-tian （Gansu University of Chinese Medicine， Key Laboratory of Dunhuang Medicine and Transformation of Ministry of Education， Provincial Key Laboratory of TCM Pharmacology and Toxicology，， Lanzhou 730000， China） 　　Abstract： Objective To observe the effects of Radix Hedysari on collagen area， hyaluronic acid （HA） and laminin （LN） of lung tissues in rat with pulmonary interstitial fibrosis； To investigate the mechanism and screen the best active ingredients in Radix Hedysari. Methods Totally 144 SPF Wistar rats were randomly divided into control group， model group， metacortandracin group， and Radix Hedysari polysaccharide， flavone and saponin groups were set as high-， medium-， and low-dose groups. Pulmonary interstitial fibrosis models were set up by dropping bleomycin into air tube of rats. All groups were taken corresponding medicine with appropriate dose daily on day 2 after models were established for 28 days. Collagen fibrils in lung tissue were observed by Masson dying and contents of HA and LN in lung tissue of rats in each group were detected by radioimmunoassay. Results Compared with the model group，