微囊藻毒素对发育12天鸭胚原始生殖细胞及其组织的影响介绍.docVIP

微囊藻毒素对鸭胚摘要：Microcystin，MC关键词：微囊藻毒素，鸭胚，of Microcystins on the Primordial Germ Cells and Tissues of Duck Embryo at 12 days CHEN YAN （Qiuzhen School of Huzhou Teachers College, Huzhou 313000） Abstract:With the development of industy and house refuse,water eutrophication is increasing day by day, cyanobacterial bloom is becoming common occurrence now.That is not only spoil the ecological environment,but also a threat to various kinds of water bird and fish.Once we ate these animals, this will cause serious harm to human body.In this study, Cherry Valley duck embryo was used as materials,we mainly used PAS staining to indentify the primordial germ cells of Duck Embryo which had developed for 12 days,and then counted the number of the primordial germ cells to study the effects of microcystin on the primordialgermcells of duck embryo.We also used paraffin-embedded tissues sections to observe the effects of microcystin on other tissues of duck embryo. Key words: Microcystins, Duck embryo, Primordial Germ Cells,Tissues 目 录 1 引言…………………………………………………………………………………1 2 材料与方法…………………………………………………………………………1 2.1实验材料………………………………………………………………………1 2.1.1 藻种 2.1.2 PAS染色试剂盒 7 载玻片的预处理 2.2实验方法和步骤………………………………………………………………4 2.2.1 微囊藻毒素的稀释 2.2.2 鸭胚的处理 2.2.3 鸭胚性腺的分离 2.2.4 PAS染色 2.2.5 石蜡组织切片的制备 2.2.6 苏木精－伊红（HE）染色 2.2.7 显微观察、照相 3 结果…………………………………………………………………………………7 3.1鸭胚处理结果…………………………………………………………………7 3.1.1鸭蛋重量 3.1.2注射微囊藻毒素及鸭胚孵化 3.1.3鸭胚胎发育的过程与特征 3.1.4鸭胚死亡率 3.2性腺PAS染色结果……………………………………………………………10 3.3切片制备结果…………………………………………………………………11 3.3.1肝脏组织切片观察 3.3.2 心脏组织切片观察 3.3.2 肌肉组织切片观察 3.3.4 腺胃组织切片观察 3.3.5 肌胃组织切片观察 3.3.6 中肾组织切片观察 3.3.7 肠组织切片观察 3.3.8 肺组织切片观察 4 讨论………………………………………………………………………………14 5 总结与展望………………………………………………………………………15 参考文献………………………………………………………………………16 致 谢………………………………………………………………………17 1. 引言微囊藻毒素主要是一种单环七肽毒素其中研究最为深入的就是具有强力肝毒性的微囊藻毒素LR[3]。在水华暴发的水体中MC大量存在，尽管MC能够被光生物降解[,5]，但在水体中的含量仍保持一定的水平，研究表明，MC在水体中可存在较长时间。MC的主要靶器官是肝脏，MC可从血液转移到肝脏。主要症状表现为肝脏充血肿大[6]，严重时可导致肝出血和坏死，致毒机理是通过抑制