蛋白酶体抑制剂对人甲状腺未分化癌细胞中PRDXs表达影响.pdfVIP

目录 一、摘要 中文论著摘要?????????????????．1 英文论著摘要?????????????????．2 二、英文缩略语??????????????????3 三、论文 前言???????????????????．4刖舌 ??????。鼍 材料与方法??????????????????5 结果???????????????????．7 讨论???????????????????．8 结论???????????????????．9 四、参考文献??????????????????．10 五、附录 综述???????????????????．．12 致谢???????????????????．．18 个人简介??????????????????．19 ·摘要· ·中文论著摘要· 蛋白酶体抑制剂对人甲状腺未分化癌细胞中PRDXs表 达的影响 目的 蛋白酶体抑制剂对人甲状腺未分化癌细胞中过氧化氧化还原蛋白 (Peroxiredoxins，PRDXs)表达的影响及其机制。 方法 选取人甲状腺未分化癌细胞，设立对照组和蛋白酶体抑制剂处理组；用实时 定量PCR法、蛋白质印迹法检测PRDXs在各组人甲状腺未分化癌细胞中的表达。 结果 与对照组相比，MGl32处理组中PRDXlmRNA和蛋白表达均显著增加(氏 0．01)、PRDX6 mRNA表达也显著增加(P0．05)，PRDX6蛋白在不同细胞系表 达水平不同，PRDX2．5 mRNA和蛋白均没有统计学差异(P0．05)。 结论 蛋白酶体抑制剂在转录水平上上调人甲状腺未分化癌细胞中PRDXl基因的 表达和上调PRDX6mRNA的表达。 关键词 蛋白酶体抑制剂；过氧化氧化还原蛋白；甲状腺未分化癌 ·英文论著摘要· The effect of proteasome inhibitor on PRDXs expression in anaplastic thyroid cancer cells Obj ective To investigate the mechanism of Peroxiredoxins 0RDXs)expression in the panel of anaplastic cancer cells affected by proteasome inhibitor． Method A panel of anaplastic cancer cells were selected for the investigation，set up control group and proteasome inhibitor treatment groups；the expression of PRDXs mRNA and protein in the panel of anaplastic cancer cells was confirmed by real-time RT—PCR and Western blot,respectively． Resuit MGl32 significantly elevated PRDXl mRNA，protein levels(尸O．01)and PRDX6 mRNA levels妒0．05)，Paradoxically，MGl 32 exposure had no stable effects on PRDX6 in the panel of anaplastic thyroid cancer cells，While PRDX2．5 had no obvious effects口0．05)when compared with control group． Conclusion Proteasome inhibitors elevated PRDXl expression at the transcriptional level and PRDX6mRNA expression． Key words Proteasome inhibitor；PRDXs；Anaplastic thyroid cancer 2 英文缩略语 英文缩写 英文诠释 中文诠释 PRDXs PCR MGl32 mRNA ECL ANOVA ROS H202 Peroxiredoxins 过氧化氧化还原蛋白 Polymerase chain reaction 聚合酶链反应 Carbobenzoxy-L-leucyl-L-leuoll-L-leucinal 一种蛋白酶体抑制剂 Messenger RNA Electrochemiluminescence Analysi S of variance Reactive oxygen species Hydrogen peroxi