高血压论文肾血管性高血压大鼠肾微血管变化的研究(精选).docVIP

高血压论文：肾血管性高血压大鼠肾微血管变化的研究 【中文摘要】为了研究肾血管性高血压大鼠肾微血管的变化,本实验采用240只SD大鼠,分为手术组和对照组,手术组又分为4周组、8周组、12周组、16周组、20周组和24周组,手术组及其相应的对照组,每组各20只。手术组通过采用实验室自制的口径为0.3mm金属套管狭窄手术组大鼠左肾动脉的方法建立2K1C肾血管性高血压大鼠模型。采用本实验室自主研制的鼠尾容积法无创血压测量系统于术前和术后分别测量大鼠的尾动脉压,分别在术后4周、8周、12周、16周、20周和24周取材,测量大鼠的肾大小,称量双肾湿重；采用墨汁明胶灌注法的各组测量肾小囊和肾小球的大小以及进行肾小球计数；用墨汁明胶灌注法和DAB法分别显示大鼠肾微血管并测算肾微血管的长度密度(Lv)、表面积密度(Sv)、体积密度(Vv),实验结果如下：1.对照组各组的血压无显著性差异(P0.05),各手术组与其对照组相比,血压均有极显著性升高(P0.05)。2.手术组大鼠左肾大小与对照组相比极显著性变小(P0.05),右肾之间也无显著性差异(P0.05)；各手术组内右肾比左肾要大,有极显著性差异(P0.05)。3.各手术组左肾重量与对照组相比极显著性减小(P0.05),右肾重量也无显著性差异(P0.05)；同一手术组内,右肾比左肾重,有极显著性差异(P0.05)。4.各手术组左肾肾小囊与对照组相比极显著性变小(P0.05)。5.各手术组左肾肾小球与对照组相比极显著性变小(P0.05)。6.显微镜下观测肾近皮质肾小球的数目,各手术组大鼠的左肾肾小球数目与对照组相比极显著性减小(P0.05),8周到24周手术组右肾小球数目与对照组相比极显著性减小(P0.05)。7.墨汁明胶灌注后的大鼠肾微血管显示为黑色的曲线状,DAB法染色后大鼠肾微血管呈棕褐色串珠状结构。与对照组相比手术组大鼠肾微血管血管形态发生了变化,肾微血管分布不均匀、连续性变差。8.墨汁明胶灌注法和DAB法显示大鼠肾微血管发现：伴随着建模时间的延长和高血压的持续作用,手术建模组的左右肾微血管的Lv、Sv、Vv与其对照组相比极显著性降低(P0.01),且左肾微血管密度始终比右肾低,有显著性差异(P0.05)；墨汁明胶灌注法的肾微血管密度低于DAB法的微血管密度。以上实验结果表明,随着建模时间的延长和高血压的持续作用,2K1C肾血管性高血压大鼠的肾形态、肾小囊和肾小球以及肾微血管都出现了损伤,肾微血管的形态和密度参数都发生了变化,肾微血管密度降低。 【英文摘要】In order to study the microvascular changes in renal vascular hypertensive Rats,240 SD (Sprague Dawley) rats were divided into operated group including 4 weeks group,8 weeks group,12 weeks group,16 weeks group,20 weeks group,24 weeks group and corresponding control with 20 in each. Renal vascular hypertensive rats named 2K1C were established by narrowing the rats?left renal arteries using self-made metal cannulas with a caliber of 0.3mm. Before and after the operation, blood pressure of each rat was measured using self-developed non-invasive measurement system by tail volume. Then, after the operation from 4 weeks to 24 weeks as described before, kidney size and fresh weight of the two kidneys of each were measured. Dimensions of renal capsules and glomeruli were measured and number of glomeruli was counted by ink and gelatin injection method. The length density (Lv), surface density (Sv) and volume density (Vv) of t