IHC与FISH检测118例乳腺浸润性导管癌HER―2的对比分析.docVIP

IHC与FISH检测118例乳腺浸润性导管癌HER―2的对比分析 　　【摘要】 目的：比较IHC与FISH检测乳腺浸润性导管癌HER-2的一致性。方法：分别用IHC和FISH对118例乳腺浸润性导管癌HER-2进行检测，比较两者检测结果的相关性。结果：118例浸润性乳腺导管癌标本，IHC检测结果为：14例为（-）；20例为（1+），55例为（2+），29例为（3+）。FISH检测结果为：53例为2.2，64例为1.8，1例为1.8-2.2。IHC检测HER-2阳性的病例FISH检测中阳性符合率96.6%（28/29），IHC检测HER-2阴性的病例FISH检测均为阴性，符合率100%（34/34）。IHC检测HER-2（2+）的55例患者中有25例经FISH检测证实HER-2阳性，30例呈阴性。FISH检测共发现5例17号染色体多体，其发生率为4.24%。IHC检测到8例乳腺癌HER-2表达存在异质性，异质性在FISH检测中亦得到证实。结论：IHC与FISH检测乳腺浸润性导管癌HER-2具有高度一致性，免疫组化HER-2（2+）病例需行FISH检测。 　　【关键词】 浸润性导管癌； HER-2； 免疫组织化学； 荧光原位杂交 　　【Abstract】 Objective：To compare the consistency of HER-2 in breast infiltrating ductal carcinoma by IHC and FISH detection.Method：HER-2 of 118 cases with breast invasive ductal carcinoma were tested by FISH and IHC detection，and compared the results correlation.Result：118 specimens of invasive ductal carcinoma were seleced， The results tested by IHC were as follows：14 cases（-）；20 cases（1+），55 cases（2+），29 cases （3+）.The results tested by FISH were as follows： 53 cases 2.2， 64 cases 1.8，1 case was 1.8-2.2.HER-2 positive coincidence rate was 96.6%（28/29） by IHC detection in positive cases by FISH detection，HER-2 negative by FISH were in negative of IHC detection， the coincidence rate was 100%（34/34）.25 HER-2 positive cases by FISH detection were among 55 cases of HER-2（2+） by IHC detection， and 30 were the negative.5 cases on chromosome multi-body in 17 were found by FISH detection，its incidence rate was 4.24%.HER-2 expression in 8 cases with of breast cancer exists heterogeneity by IHC detection，and the heterogeneity was also confirmed in FISH detected.Conclusion：HER-2 of breast infiltrating ductal carcinoma by IHC detection was high consistency with FISH detection，HER-2 cases（2+） by immunohistochemical need to be detected by FISH. 　　【Key words】 Infiltrating ductal carcinoma； HER-2； Immunohistochemistry； Fluorescence in situ hybridization 　　正确检测和评定乳腺癌的HER-2蛋白表达和基因扩增状态对乳腺癌的临床治疗和预后判断至关重要，HER-2检测结果不仅涉及患者的预后评估，而且是筛选是否适合针对HER2的靶向治疗的先决条件，并且可以对内分泌治疗、化疗