T辅助淋巴细胞的功能变化在急性肺损伤中的意义研究.docVIP

T辅助淋巴细胞的功能变化在急性肺损伤中的意义研究 　　【摘要】 目的：探讨T辅助淋巴细胞的功能变化在急性肺损伤中的意义。方法：选取100只小鼠作为研究对象，采用随机数字表法将其分成观察组和对照组，每组50只。观察组小鼠注射适量脂多糖制作急性肺损伤模型，通过酶联免疫吸附测定小鼠细胞清液内的干扰素及白细胞介素含量，采用逆转录-PCR测定方法对小鼠IFN-γ基因表达情况进行检测，观察比较两组测定结果。结果：观察组小鼠上清液中IFN-γ的含量明显低于对照组，IL-4含量明显高于对照组，两组比较差异有统计学意义（P0.05），观察组小鼠体内脾细胞中IFN-γ基因表达能力明显低于对照组，两组比较差异有统计学意义（P0.05）。结论：当发生急性肺损伤后，小鼠会产生明显的抗炎症反应，T辅助淋巴细胞的功能变化在急性肺损伤的发生发展中作用较大。 　　【关键词】 T辅助淋巴细胞； 功能变化； 急性肺损伤； 意义研究 　　【Abstract】 Objective：To investigate the significance of functional changes of T helper lymphocyte in acute lung injury.Method：100 mice were selected and randomly divided into the observation group and the control group，each group had 50 mice.Mice in the observation group were injected with proper amount of LPS and made models of acute lung injury.The contents of interferon and interleukin in the cell supernatants of mice were measured by enzyme linked immunosorbent assay.The expression situation of IFN-γ gene in the mice’s spleen cells was measured by reverse transcriptase-PCR determination method.The measurement results were compared between the two groups.Result：The content of IFN-γ in the observation group was lower than that in the control group，the content of IL-4 in the observation group was higher than that in the control group，the differences were all statistically significant（P0.05）.The expression ability of IFN-γ gene in the observation group was lower than that in the control group，the difference was statistically significant（P0.05）.Conclusion：When the acute lung injury occurs the mice will produce anti inflammatory response.The functional changes of T helper lymphocytes have great effect on the incidence and development of acute lung injury. 　　【Key words】 T helper lymphocyte； Functional change； Acute lung injury； Significance research 　　First-author’s address：The First People’s Hospital of Guangzhou City，Guangzhou 510180，China 　　doi：10.3969/j.issn.1674-4985.2015.25.005 　　目前认为，促炎与抗炎反应失衡为诱导急性肺损伤发生的根本原因，但目前对促炎与抗炎反应失衡准确评估有较大难度。大量研究表明，T辅助淋巴细胞可反映机体炎症反应