奥沙利铂对原发性肝癌细胞系HUH―7的抗肿瘤效果评价.docVIP

奥沙利铂对原发性肝癌细胞系HUH―7的抗肿瘤效果评价 　　[摘要] 目的 探讨奥沙利铂对原发性肝癌细胞系HUH-7是否会产生作用和影响，根据其对抗肿瘤的效果决定是否能够应用于对肝癌细胞的临床治疗。 方法 把中科院2012年3月―2014年4月的肝癌细胞作为该试验的研究对象，用流式细胞仪器对癌细胞的分布周期和死亡状况进行分析，运用MTT法测试不同浓度和不同的作用时间的奥沙利铂对肝癌细胞增长繁殖的抑制效果。通过多次试验，得出最终结论。 结果 经过48 h作用后，癌细胞死亡率达到了11.8%，可见奥沙利铂对于肝癌细胞的繁衍增长有着很强的抑制功效，并且呈现出计量和时间的依赖性。结论 奥沙利铂对原生性肝癌细胞系HUH-7的抗瘤效果明显，它可以抑制肝癌细胞繁殖，使细胞周期停留在S期和G2PM期之间，进而使癌细胞死亡。但是其具体的运行机制还有待研究，因而奥沙利铂能否应用于临床治疗还不能盖棺定论。 　　[关键词] 奥沙利铂；原发性；肝癌细胞；效果 　　[中图分类号] R735 [文献标识码] A [文章编号] 1674-0742（2015）02（c）-0089-03 　　[Abstract] Objective To observe the effect of oxaliplatin on hepatocellular carcinoma cell line HUH-7 will produce the effect and influence， according to the anti - tumor effect decided whether to apply in the clinical treatment ofhepatocellular carcinoma cells. Methods Hepatoma cells as the object of study in this experiment， the distribution of cancer cell cycle were analyzed by flow cytometry instrument and death situation， using MTT method to test the inhibitory effect of different concentrations and different action time of oxaliplatin on hepatocellular carcinoma cell growth and reproduction of.Through many experiments， and draw the final conclusion. Results After 48 hours after the action of cancer cell death rate reached 11.8%， visible Asha Leigh Per has a very strong inhibitory effect on liver cancer cells multiplygrowth， and presents the measurement and time dependence. Conclusion Anti tumoreffect conclusion oxaliplatin on primary hepatocellular carcinoma cell line HUH-7 is obvious， it can inhibit the hepatoma cell reproduction， so that the cell cyclehere between the S phase and G2PM phase， and then make the death of cancer cells. But the specific operation mechanism remains to be studied，which could be used in the clinical treatment of oxaliplatin is not conclusive. 　　[Key words] Asha Leigh Per； Primary； Hepatocellular carcinoma cell； Effect 　　原发性肝癌对人身体危害极大，且不易治愈。传统的治疗方法以手术为主，化疗为辅。但是，这一治疗方式有很大不足之处。有很多肝癌患者发现自己的病情、进行治疗时肝癌已经发展到晚期，不能进行手术，因为此时的病人的体制已经太差，经不起手术的折腾。而且，近几年肝癌患者在经过