调节性T细胞及其细胞因子在弱精子症患者的表达与临床意义.docVIP

调节性T细胞及其细胞因子在弱精子症患者的表达与临床意义 　　【摘要】 目的：观察CD4+CD25+调节性T细胞（Treg）在弱精症不育患者变化规律以及与精子质量的相关性。方法：收集60例弱精症不育患者为观察组及36例健康志愿者为对照组，收集其外周血及前列腺液标本，采用流式细胞术检测外周血及前列腺液内（Treg）及其胞内转化生长因子β1（TGF-β1）及白介素10（IL-10）含量，并分析以上三者与精子活力及活率的相关性。结果：观察组患者外周血及前列腺按摩液Treg、IL-10及TGF-β1均显著低对照组（P0.075，P0.05），观察组精子活率、运动速度和ATP含量指标均显著低于对照组，两组比较差异有统计学意义（P0.05）；经Pearson检验，CD4+CD25+ Treg、TGF-β1和IL-10与精子活率、畸形率、运动速度、精子ATP指标之间均呈正相关性。结论：CD4+CD25+ Treg免疫异常所致的精子的免疫性破坏过度，可能是弱精症不育症的重要免疫学机制。 　　【关键词】 调节性T细胞； 弱精症； 免疫破坏； 免疫抑制细胞因子 　　【Abstract】 Objective： To observe the change pattern of CD4+CD25+ regulatory T cells （Treg） in asthenospermia infertile patients and the correlation with sperm quality.Method： The peripheral blood and prostatic fluid specimens were collected from 60 asthenospermia infertile patients as the observation group and 36 healthy volunteers as the control group. The flow cytometry was used to determine the volume of Treg， intracellular transforming growth factor β1 （TGF-β1） and interleukin 10 （IL-10） in the peripheral blood and prostatic fluid.The correlation of the above three substances of sperm viability and motility rate were analysed.Result：Compared with control group，the observation group patients of Treg， IL-10 and TGF-β1 asthenospermia infertile had significantly lower volume in their peripheral blood and prostate massage fluid，the differences were statistically significant（P0.075，P0.05）.There was no significant difference in the sperm volume of the two groups. The sperm motility rate， sperm velocity and ATP of the observation group were all obviously lower than those of the control group， the difference was statistically significant（P0.05）. The Pearson method was used， CD4+CD25+ Treg，TGF-β1 and IL-10 had positive correlation with the index of sperm motility rate， malformation，velocity，and ATP.Conclusion：The dysimmunity of CD4+CD25+ Treg， which causes excessive damage to the sperm immunity， is possibly an important immunological mechanism of the asthenospermia infertility. 　　【Key words】 Re