树头菜黄酮提取工艺优化及体外抗氧化活性.docVIP

树头菜黄酮提取工艺优化及体外抗氧化活性 　　摘要：以80%乙醇为提取溶剂，以树头菜（Crateva unilocalaris Buch.）黄酮提取率为指标，在单因素试验基础上，用响应面法优化野生树头菜黄酮的提取条件，并测定其体外抗氧化活性。结果表明，最佳提取工艺为提取温度65 ℃、提取时间1.3 h、液料比28∶1（mL∶g）、提取2次，树头菜黄酮提取率为0.497%。树头菜黄酮具有较强的清除超氧阴离子自由基、羟自由基的能力和还原能力，清除超氧阴离子自由基和羟自由基的IC50分别为0.615、0.415 mg/mL，其抗氧化能力高于抗坏血酸（VC）和叔丁基对苯二酚（TBHQ）。 　　关键词：树头菜（Crateva unilocalaris Buch.）；响应面法；黄酮；抗氧化活性 　　中图分类号：S647；Q819 文献标识码：A 文章编号：0439-8114（2014）13-3145-04 　　Optimizing Extraction of Flavonoids from Crateva unilocalaris Buch. and Its 　　Anti-oxidant Activity in Vitro 　　LIU Yu-fen，XIA Hai-tao，YAN Wei-wei 　　（College of Chemical Engineering， Huaihai Institute of Technology， Lianyungang 222005， Jiangsu， China） 　　Abstract： Using 80% ethanol as extraction solvent， the extraction ratio of flavonoids from Crateva unilocalaris Buch. as index， response surface methodology was used to optimize the extraction of flavonoids from Crateva unilocalaris Buch. with single factor experiment. The antioxidant activity in vitro was evaluated by total reducing power assay. The results showed that the optimal extraction conditions were extraction temperature of 65 ℃， extraction time of 1.3 h， liquid-to-material ratio of 28∶1（mL∶g） and repeated extraction number of 2. Under these conditions， the extraction ratio of flavonoids was up to 0.497%. Crateva unilocalaris Buch. flavonoids had excellent reducing power and high abilities to scavenge superoxide anion and hydroxyl free radicals with IC50 values of 0.615 mg/mL and 0.415 mg/mL， respectively. The antioxidant effect of flavonoids from Crateva unilocalaris Buch. were higher than that of VC and TBHQ. 　　Key words： Crateva unilocalaris Buch.； response surface methodology； flavonoids； antioxidant activity 　　树头菜（Crateva unilocalaris Buch.）是一种营养丰富、口味独特的野生蔬菜。树头菜是山柑科鱼木属的一种乔木，主要分布于海拔1 500 m以下的热带、亚热带地区[1，2]，生长于平地或山谷林地等，多为野生[3]。它不仅可作为行道绿化树种，还兼具药食价值，其根、茎、叶均可入药，味苦性寒。根具有清热解毒、舒筋活络的功效，可治疗肝炎、痢疾等疾病；叶具有健胃的功效；可解毒，治烂疮[4-7]。树头菜含有氨基酸、维生素、矿物质、黄酮、糖类等多种生物活性成分[8-10]。目前，对树头菜的研究主要以化学成分及分析方法为主。黄酮是一种天然的生物活性物质，具有药理作用和