花生红衣对巨核细胞——血小板调控研究进展-第三军医大学学报.docVIP

单宁酸对巨核细胞损伤的保护作用 （400038 重庆，第三军医大学军事预防医学院全军复合伤研究所，创伤、烧伤与复合伤国家重点实验室） 目的研究单宁酸(tannic acid，TA)对巨核细胞损伤的保护作用，并探讨其作用机制。方法?体外培养巨核细胞株M07e，经0、0.5、1、2.5、5、10 μg/m单宁酸预处理后给予10 Gy 60Co γ射线照射，继续培养不同时间，采用MTT法、荧光酶标仪检测法和流式细胞检测技术分别检测细胞存活率、活性氧（ROS）水平、线粒体膜电位(mitochondrial membrane potential，MMP)和凋亡率变化。给予B/c小鼠5 Gy 60Co γ射线全身一次性照射，复制急性放射损伤实验动物模型，设置生理盐水对照组和TA灌胃预处理组，通过尾静脉外周血常规检测和骨髓病理切片观察，分析血小板水平和骨髓巨核细胞数目变化情况。结果TA能够剂量依赖性地提高受照巨核细胞的存活率；与单纯辐照组相比，TA预处理可以显著降低细胞内总ROS水平（0.01）、提高细胞线粒体膜电位（0.05）和降低细胞凋亡率（0.05）。动物实验结果显示，TA预处理能够显著抑制辐射损伤引起的骨髓巨核细胞数量减少，照后第7、12、15、19天时TA处理组血小板水平显著高于对照组（0.05，0.01）。结论TA对巨核细胞损伤具有显著保护作用，其作用机制可能与其突出的抗氧化应激活性有关。 单宁酸；巨核细胞；血小板；辐射损伤 Tannic acid protects megakaryocytes against ionizing radiation via its anti-oxidative activity Wu Sunan, Xu Yang, Chen Shilei, Shen Mingqiang, Chen Fang, Chen Mo, Zhang Zhou, Wang Song, Su Yongping, Cheng Tianmin, Wang Junping (State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Military Preventive Medicine, Third Military medical University, Chongqing, 400038, China) [Abstract] Objective To determine the protective effect of tannic acid (TA) on megakaryocytes against ionizing radiation and investigate its underlying mechanism. Methods Human megakaryoblast cell line M07e cells were pretreated with TA at concentrations of 0, 0.5, 1, 2.5, 5 and 10 μg/mL before receiving 10 Gy γ-ray irradiation. The cell viability, levels of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and apoptotic rate of the cells were detected after irradiation. Subsequently, BALB/c mice with or without TA treatment were irradiated with 60Co γ at a dose of 5 Gy, and the blood platelet count and number of megakaryocytes in bone marrow were determined at appointed time points. Results TA dose-dependently improved the survival rate of M07e cells after irradiation. Compared with control group, TA significantly reduced the intracellular ROS levels (P0.05), impro