Aflatoxins.pptVIP

Food Technology U.C.C. Aflatoxin What ? Causes? Harm In Food? Standard? Determination? Control? In the 1960 more than 100,000 young turkeys on poultry farms in England died in the course of a few months from an apparently new disease that was termed Turkey X disease Structure and feature 双呋喃环-toxic 氧杂奈邻酮-cancer B1, B2,purple G1, G2,green M1, M2, GM1, 熔点范围为200～300C，其结构式中一般都有内酯环，当内酯环被打开时，荧光消失，毒性消除。内酯环易与氧化剂反应，特别是与碱性试剂反应，可部分分解为酚式化合物。黄曲霉毒素可 与NaOH pH9 溶液生成钠盐，使荧光消失。 365nm colour Toxicity 黄曲霉和寄生 曲霉产生的杂环化合物 LD50=0.62mg/kg(pig), LD50AFB1=0.36 mg/kg(pig)较敌敌畏大100 倍，较砒霜大60 倍，较氰化钾大10 倍 Health risk Liver inflammation (hepatitis) Immune deficiency Increased susceptibility to infections Cirrhosis of the liver Liver cancer Aflatoxin MLs GB2761-2005 Sources Aspergillus flavus黄曲霉 A. parasiticus寄生曲霉 (A. nom ius)集峰曲霉菌 Sources ·? Peanuts ·??Whole grains · Nuts, especially older nuts (they taste stale), and those in grains ·??Non-human grade foods · Commercial diets that contain higher levels of cottonseed oil, fish meal, and peanut oil Foods from countries that have substandard storage elevators for grains Analysis 薄层色谱法(thin-layer chromatography , TLC) GB50059.22-85(365nm) AOAC INTERNATIONAL 酶联免疫吸附法( enzyme-linked immunosorbent assay , ELISA) 高效液相色谱法(high performance liquid chromatography , HPLC) Analysis--HPLC Nutrition Status and Aflatoxin Metabolites in Urine and Feces Among Male College Students in Guangzhou Nutrition Status and Aflatoxin Metabolites in Urine and Feces Among Male College Students in Guangzhou Nutrition Status and Aflatoxin Metabolites in Urine and Feces Among Male College Students in Guangzhou Surve illance on con tam ina tion of tota l afla tox in s in corn, peanut, r ice, wa lnut and p ine nut inseveral area s in China Peanut Rice, Walnut and pinenut Aflatoxin- reversed-phase High pressure Liquid Chromatography Sample milling--Extraction with acetonitrile/water - filtrated- purified with MycoSep TM colum