Cloning of a Novel Arylamidase Gene from Paracoccus sp..pptVIP

Cloning of a Novel Arylamidase Gene from Paracoccus sp. Strain FLN-7 That Hydrolyzes Amide Pesticides First group of special english 王燕 李庆杰 王莹 尤晴 闫嘉琪 左冰 张钟文 周志勤 Paracoccus sp.: Hydrolyzes amide pesticides through amide bond cleavage. (diflubenzuron, propanil,chlorpropham,and dimethoate et al.) ampA: (subclone) Encoding a novel arylamidase that catalyzes the amide bond cleavage. 1395-bp ORF encode a 465-aminoacid protein. Expressed in E.coli BL21, Purified using Ni-nitrilotriacetic acid affinity chromatography. Homodimer ( isoelectric point of 5.4).