阿萨希毛孢子菌氟康唑体外诱导耐药的方法优化.docVIP

阿萨希毛孢子菌氟康唑体外诱导耐药的方法优化 张德全1，2 ，夏志宽2 ，田艳丽2 ，廖勇2 ，吕雪莲2 ，陈卫2，王聪敏2，杨蓉娅2(400038 重庆，第三军医大学1；100700 北京，北京军区总医院全军皮肤损伤修复研究所2) [摘要(Trichosporonasahii，T.asahii)获得 [关键词]阿萨希毛孢子菌；氟康唑；耐药性；最小抑菌浓度 [中图法分类号] [文献标志码]A Optimization on induction offluconazole resistance in vitro inTrichosporonasahii ZHANG De-quan1，2，XIA Zhi-kuan2，TIAN Yan-li2，LIAO Yong2，LU Xue-lian2，CHEN Wei2，WANG Cong-min2，YANG Rong-ya2 (1. 400038，Chongqing，Third Military Medical University;2. 100700，Beijing，The Military Institute of Injury and Reparation，General Hospital of Beijing Military Command of PLA) [Abstract]Objective To compare thesuccessful rate and stability of fluconazole induction resistant strains in T.asahii, which were induced by fluconazole alone,combined with methylprednisolone, and methylprednisolone alone. Methods 16clinical and environmentalstrains ofT.asahiiwere tested the susceptibility againstfluconazole in vitro, and wereinduced resistance to fluconazole by fluconazole alone,fluconazole combinated with methylprednisolone and methylprednisolone alonerespectively,comparing with the control group.The resistant strains were passed 30 times in drug-free medium to assess the stability of resistance.Results The negative control?group and?methylprednisolonealone group?had no obvious?changes in? MIC during the induction.12 resistant strains(75%) were obtained by?fluconazole?alone induction group, and 16 strains(100%)?were obtainedbyfluconazolecombinedmethylprednisolone?group.After 30?passages,?fluconazole?resistant strains?(37.5%)?were maintained in fluconazole induced group,and 9 fluconazole?resistant strains?(56.25%) were?maintained?in fluconazole combined methylprednisolonegroup.Conclusion Methylprednisoloneis helpful to increase the?successful?rate and stability of T.asahii resistant strains?induced by fluconazole in vitro,fluconazole combinedmethylprednisolone induction is superior to fluconazole alone inT. asahii?fluconazole resistance model construction in vitro. [Key