大鼠心肌梗死后血浆Ghrelin的动态变化.docVIP

大鼠心肌梗死后血浆Ghrelin的动态变化 　　[摘要] 目的 探讨大鼠心肌梗死后血浆Ghrelin水平改变及与心功能改变的关系。 方法 30只Wistar大鼠被随机分成对照组（n=6只），假手术组（n=6只），心肌梗死3 d组（n=6只），梗死7 d组（n=6只）和梗死28 d组（n=6只）。通过结扎冠状动脉前降支制作心肌梗死动物模型，假手术组开胸后仅在冠状动脉下穿线，但不结扎。血流动力学检查记录心率（HR）、左心室收缩压（LVSP）、左心室舒张末期压力（LVEDP）以及压力上升最大速率（dp/dtmax）。对各组动物采集外周血并制备血清，采用ELISA方法检测血浆Ghrelin浓度。 结果 与假手术组相比，梗死组大鼠LVSP和dp/dtmax显著降低（P 0.05）。 结论 心肌梗死后大鼠血浆Ghrelin浓度改变可能与梗死后心脏重构相关。 　　[关键词] 心肌梗死；Ghrelin；心力衰竭；大鼠 　　[中图分类号] R65.1 [文献标识码] A [文章编号] 1673-7210（2013）11（a）-0011-03 　　Dynamic change of plasma Ghrelin levels in rats with myocardial infarction 　　YUAN Mingjie QUAN Li KONG Bin WANG Shaobo TANG Yanhong HUANG He 　　Department of Cardiology， Renmin Hospital of Wuhan University， Hubei Province， Wuhan 430060， China 　　[Abstract] Objective To observe the correlation between cardiac function and the dynamic expression of Ghrelin from 3 days to 28 days in rats with myocardial infarction. Methods 30 Wistar rats were divided into four groups by the way of random number. They were control group （n=6）， sham-operated group （n=6）， MI-3 d group （n=6）， MI-7 d group （n=6） and MI-28 d group （n=6）. The left anterior descending （LAD） was ligated and the sham animals underwent thoracotomy and pericardiotomy， but not LAD ligation. All the animals in each groups were underwent cardiac catheterization， include heart rate， left ventricular systolic pressure， left ventricle end-diastolic pressure and dp/dtmax. The concentration of Ghrelin in plasma was measured by using enzyme-linked immunosorbent assay （ELISA）. Results Compared with control group and sham-operated group， the LVSP and dp/dtmax at each MI-groups was markedly decreased （P 0.05）， but LVEDP was increased （P 0.05）. compared with the MI-3 d and MI-7 d group， LVEDP was increased in MI-28 d group （P 0.05）. The expression of Ghrelin was dynamic reduction in rats with myocardium infarction （P 0.05）. There was no different of Ghrelin level in sham-operated group and control group. Conclusion This change of Ghrelin expression ma