紫癜性肾炎患儿血清IgA1水平的测定与分析.docVIP

紫癜性肾炎患儿血清IgA1水平的测定与分析 　　[摘要] 目的 通过测定并比较紫癜性肾炎（HSPN）患儿及健康儿童血清IgA1水平，探讨IgA1对HSPN发病的影响。 方法 收集2013年5~12月河南中医学院第一附属医院儿科住院的HSPN患儿40例（HSPN组）及20名健康儿童（对照组）献血者血清，采用免疫比浊法测定HSPN患儿及健康儿童血清IgA的浓度；通过Jacalin亲和层析法分离、纯化血清，得到Jacalin结合蛋白，测定两组血清总IgA1浓度；Jacalin结合蛋白通过分子筛层析法分离，测定两组单聚体IgA1（mIgA1）及多聚体IgA1（pIgA1）的浓度。 结果 HSPN组及对照组血清IgA水平比较，差异无统计学意义（P 0.05）；HSPN组血清总IgA1、mIgA1及pIgA1水平均高于对照组，差异有高度统计学意义（P 0.01）。 结论 HSPN患儿血清IgA水平升高不明显，血清中mIgA1及pIgA1水平明显升高，提示IgA1在HSPN的发病中起一定作用。 　　[关键词] 紫癜性肾炎；IgA1；亲和层析；分子筛层析 　　[中图分类号] R725.9[文献标识码] A[文章编号] 1673-7210（2014）06（b）-0013-04 　　 　　Measurement and analysis of serum IgA1 level in children with henoch-schonlein purpura nephritis 　　CUI Yafan1 DING Ying2 　　1.Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100700, China; 2.Department of Pediatrics, the First Affiliated Hospital of Henan University of TCM, Henan Province, Zhengzhou 450003, China 　　[Abstract] Objective To measure and compare the differences of IgA1 serum level in children with henoch-schonlein purpura nephritis (HSPN) and healthy children (control group), and discuss the impact on the disease. Methods Serums of children with HSPN (HSPN group) and healthy children from May to December 2013 in the First Affiliated Hospital of Henan University of TCM were collected. Concentration of serum IgA in two groups were measured by immunoturbidimetry; Jacalin bound protein was separated and purified by Jacalin affinity chromatography, and concentration of total serum IgA1 in two groups were measured; monomeric IgA1 and polymeric IgA1 were separated by molecular sieve chromatography and the concentration of mIgA1 and pIgA1 of two groups were measured. Results There was no statistically significant difference of two groups in the concentration of serum IgA (P 0.05); the concentration of total serum IgA1, mIgA1 and pIgA1 in children with HSPN was significantly higher than those of the control group, the difference was high statistically significant (P 0.01). Conclusion The incr