线栓法建立局灶性脑缺血模型的方法与探讨.docVIP

线栓法建立局灶性脑缺血模型的方法与探讨 　　[摘要] 目的 探讨成功建立大鼠局灶性脑缺血模型的有效方法及影响因素。 方法 将大鼠分为250～300 g和350～400 g两个体重组。为观察禁食对造模的影响，250～300 g体重组又分为对照组（n=10）和禁食组（n=10）；为观察栓线直径对造模的影响，350～400 g体重组又分为细栓线组（n=10）和粗栓线组（n=10）。栓线经颈总动脉、颈内动脉进入大脑中动脉的起始部位阻断大脑中动脉的血流。术后通过神经功能缺损评分和氯化三苯基四氮唑（TTC）染色对该模型进行评价。 结果 在250～300 g体重组，造模成功率高，禁食组死亡率（11.1%）明显低于对照组（62.5%）（P 0.05）；在350～400 g体重组，死亡率低，粗栓线组造模成功率（80%）明显高于细栓线组（20%）（P 0.05）。 结论 术前禁食可降低动物死亡率，根据大鼠体重选择相应直径的栓线可提高造模的成功率。 　　[关键词] 栓线法；局灶性脑缺血；禁食；栓线直径 　　[中图分类号] R363.1 [文献标识码] A [文章编号] 1673-7210（2014）08（c）-0033-03 　　The method and explore of establishing focal ischemia model with bolt line 　　JIA Shuwei1 XIA Qing2 　　1.Department of Physiology， Harbin Medical University， Heilongjiang Province， Harbin 150086， China； 2.Institute of Acupuncture， Tianjin Chinese Medical University， Tianjin 300193， China 　　[Abstract] Objective To investigate the method and influence factors of establishing focal ischemia model of rat successfully. Methods Rats were divided into two groups， 250-300 g group and 350-400 g group. In order to observe the influence of fasting on the model， 250-300 g group was subdivided into two groups， control group （n=10） and fasting group （n=10）. In order to observe the influence of the bolt line diameter on the model， 350-400 g group was sub-divided into two groups， thin bolt line group （n=10） and bold bolt line group （n=10）. The bolt line entered from a cut of the common carotid artery and moved in the intracranial internal carotid artery and at last reached to the starting positions of middle cerebral artery to block blood flow. The nerve function defect score and TTC staining were used to evaluate the model. Results In the 250-300 g group， the success rate was high and mortality of the fasting group （11.1%） was lower than the control group （62.5%） （P 0.05）. In the 350-400 g group， mortality was low and the success rate of the bold bolt line group （80%） was higher than the thin bolt line group （20%） （P 0.05）. Conclusion Fasting can reduce animal morta