分化抑制因子ndpka含量与血液病的相关性研究.docVIP

分化抑制因子NDPKA含量与血液病的相关性研究 nbsp;nbsp;nbsp;nbsp;【关键词】nbsp; 白血病 【摘要】 目的 研究分化抑制因子―――核苷二磷酸激酶A（NDPK-A）在血清中的含量及其与血液病的相关性，探讨血清NDPK-A含量检测对于血液病诊断和疗效判断的意义。方法 应用重组NDPK-A标准品及相应抗体建立双抗体夹心ELISA法测定NDPK-A含量，应用此方法测定正常人和血液病患者血清NDPK-A含量。结果 30例正常人血清NDPK-A含量为（4.22±0.45）μg/L，26例白血病患者血清NDPK-A含量为（21.07±12.03）μg/L，9例淋巴瘤患者血清NDPK-A含量为（32.31±8.75）μg/L，8例骨髓瘤患者血清NDPK-A含量为（14.80±5.87）μg/L。白血病患者治疗前后血清NDPK-A含量差异显著，14例治疗前患者NDPK-A含量为（31.25±10.35）μg/L，治疗后患者NDPK-A含量为（12.17±4.16）μg/L。结论 建立的双夹心ELISA法可以灵敏、准确地测定血清NDPK-A含量;NDPK-A含量检测可发展为白血病的早期诊断和疗效判断指标。 关键词 核苷二磷酸激酶A 白血病 双抗体夹心酶联免疫吸附测定法 . 【Abstract】 Objective To investigate the relationship between the serum NDPK-A level and the development of leukemia，and the significance of NDPK-A determination in leukemia diagnosis. Methods A sandwich ELISA，established on the basis of recombinant human NDPK-A and its antibodies，was employed to detect serum NDPK-A concentration in healthy people and patients with hematological malignancies. Results Serum NDPK-A concentration was 4.22±0.45μg/L among 30 healthy volunteers. It was 21.07±12.03μg/L among 26 leukemia patients，and 32.31±8.75μg/L among 9l ymphoma patients，and 14.80±5.96μg/L among 8 myeloma patients. Furthermore，there was significant difference between untreated and treated leukemia patients. Serum NDPK-A concentration was 31.25±10.35μg/L among 14 untreated patients，and 12.17±4.16μg/L among 12 treated patients. Conclusion The ELISA used here was simple，sensitive and accurate for determination of serum NDPK-A level，which might be developed into an early diagnosis and prognostic factor of leukemia. Key words NDPK（nucleoside diphosphate kinase）-A leukemia ELISA 核苷二磷酸激酶A（NDPK-A）是抑癌基因nm23-H1的编码产物，除了能够催化NDP和NTP之间的磷酸基转移反应外，还可以调节细胞增殖、分化、发育和凋亡 ［1，2］ 。多项研究表明，NDPK-A的表达量与多种肿瘤的转移潜能呈负相关，这一关系在肺癌、膀胱癌、乳腺癌、胃癌、卵巢癌等恶性肿瘤患者中尤为明显 ［3，4］ 。另外，NDPK-A可以抑制血细胞分化，最近有研究报道，血清NDPK-A含量与白血病的预后相关，高NDPK-A含量的白血病患者预后差 ［5，6］ 。为了探讨血清NDPK-A含量检测对于白血病早期诊断和疗效判断的意义，笔者建立了血清NDPK-A含量双抗体夹心ELISA检测方法，并检测了部分血样，现报告如下。 1 材料与方法 1.1 主要仪