载锌沸石对蛋鸡免疫和抗氧化功能的影响.docVIP

载锌沸石对蛋鸡免疫和抗氧化功能的影响 摘 要 本文以载锌沸石为研究对象，以产蛋高峰期蛋鸡为试验动物，研究载锌沸石对蛋鸡生产性能、血清激素水平、血清指标、肠道菌群、血清、组织抗氧化和免疫功能、金属硫蛋白及抗氧化相关基因表达的影响，为进一步研究载锌沸石的作用机理及其替代抗生素的可能性提供理论依据。论文由3个试验组成： 试验一，随机选取648只31周龄蛋鸡分为6组，每组6个重复，每个重复18只。对照组饲喂基础日粮中添加ZnSO4的对照日粮(锌添加量为80 mg/kg)，抗生素组在上述对照日粮中添加40 mg/kg杆菌肽锌，试验1、2、3、40.46%载锌沸石组可提高血清雌二醇(E2)水平(P0.05)。0.46%载锌沸石组显著增加试验第4周时血清中葡萄糖含量(P0.05)，0.23%、0.34%、0.46%载锌沸石使血清胆固醇、甘油三酯（TG）（BUN）TG含量(P0.05)。与抗生素组相比，0.46%载锌沸石组显著提高了血清中E2水平和试验第8周血清中促黄体素水平(P0.05)，日粮中添加0.34%、0.46%载锌沸石使血清中BUN含量显著降低(P0.05)。日粮中添加载锌沸石对蛋鸡肠道菌群没有显著影响(P0.05)。从试验一结果来看，0.23%、0.34%、0.46%载锌沸石组效果优于其他组。 试验二研究载锌沸石对血清、组织抗氧化和免疫功能的影响。研究发现0.46%载锌沸石组蛋鸡肝脏铜锌超氧化物歧化酶（CuZn-SOD）活性、总超氧化物歧化酶（T-SOD）活性显著高于对照组(P0.05)，0.23%、0.34%、0.46%载锌沸石组肝脏过氧化氢酶(CAT)活性、谷胱甘肽过氧化物酶（GSH-Px）活性显著提高(P0.05)，0.34%载锌沸石组在试验第4周和8周时血清T-SOD活性显著高于对照组(P0.05)，而0.34%、0.46%载锌沸石组分别在试验第8周时血清丙二醛（MDA）EFFECT OF ZINC-BEARING ZEOLITE ON IMMUNITY AND ANTIOXIDANT FUNCTION OF LAYING HENS ABSTRACT In this paper, zinc-bearing zeolite as the research object, hens of fastigium laying period as experimental animals, to evaluate the effect of zinc-bearing zeolite on production performance, serum hormones, serum parameters, enterobacteria, serum and tissue antioxidant and immunity function, and related gene expression. The study is divided into three trials and the results are as follows : Trial 1: 648 31-week-old laying hens were divided into six groups with six replicates of 18 birds. The dietary treatments were as follows: (1) basal diet supplemented with Zn-sulfate (80 mg/kg from ZnSO4); (2) the control diet supplemented with antibiotics (40 mg/kg of bacitracin zinc); (3) a ZnSO4-deficient diet supplemented with zinc-bearing zeolite (ZnBZ) at level of 0.11%, 0.23%, 0.34% and 0.46% (supplying 20, 40, 60, 80 mg/kg of Zn), respectively. The trial was conducted to study the effects of ZnBZ on production performance, level of hormones serum parametersand enterobacteria of laying hens. The results indicated that 0.23%, 0.34% and 0.46% ZnBZ decreased broken eggshell rate compared with t