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GHS ScienceCAPT Embedded Task Review Lab Investigation: Yeast Population Dynamics * Introduction Why Study Yeast Populations? Yeast is a good model organism because it is simple, but has many of the same processes as more complicated cells. Yeast use simple sugars to perform cellular respiration and make ATP. During cellular respiration, they release CO2 as a waste product, just like animals and plants. [sugar + O2 ? CO2 + H2O + ATP (energy)] Since more yeast cells will release more CO2, we can use the amount (or volume) of CO2 volume as a measurement of the amount of yeast cells. The amount of yeast cells will tell us the size of the population. [Amount of CO2 ~ Population Size] DID YOU KNOW? Yeast population variations produce flavor characteristics in a wide variety of food products such wine, beer, and bread. Use of Energy, Growth and Reproduction in Yeast Yeast Cell ADD SUGAR The yeast cells undergo cellular respiration! As they grow, more cells make more CO2! Cell Division Occurs SUGAR The process repeats producing more yeast cells and even more CO2 gas as more cells make more gas Your Task Determine how environmental conditions such as pH, temperature, light, etc. can effect the growth of a yeast population REMEMBER: we can use CO2 (carbon dioxide) volume as a measurement of the size of the yeast population in the test tube. Measuring Yeast Population Growth by CO2 Production Next, add 1ml of yeast mixed in water Fill a small test tube with 35 ml of a 25% Molasses solution. Mix the yeast in the solution by putting the clean palm of your hand over the end and inverting it 5 times CO2 Collection Set-up This is the double test tube set up for collection of CO2. To obtain this the lager test tube is placed over top of the smaller filled test tube. It is then quickly inverted so that the larger test tube is open and the smaller one is upside down. An air bubble will be left at the top of the tube. Large Test Tube S