absorbance measurementswith ocean optics u s b- i s s- u v v i s（吸光度measurementswith海洋光学u sb -我s s - u v v）（5页）.docVIP

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2017-01-19 发布于湖南
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absorbance measurementswith ocean optics u s b- i s s- u v v i s（吸光度measurementswith海洋光学u sb -我s s - u v v）（5页）.doc

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Ocean Optics USB-ISS-UV/VIS Spectrometer and OOIBase32 Software To take an absorbance measurement: Connect the power supply to a standard power outlet, and then connect the 5-volt power connector to the USB-ISS-UV/VIS. This will activate the unit, and the fan should begin to spin. Connect the USB cable to the USB2000 Spectrometer and to the PC. The USB-ISS-UV/VIS turns both the UV and VIS lamps on by default when power is connected to the unit. However, the UV lamp may take up to two minutes to start, depending on how long it has been inactive. Open the OOIBase32 software. Select Strobe Enable from the OOIBase32 Acquisition Parameters menu bar to open the internal shutter on the USB-ISS-UV/VIS. Enter the Scope Mode: Click the Scope Mode icon on the toolbar. Make sure the signal is on scale. The peak intensity of the reference signal should be about 3500 counts. If necessary, adjust the integration time until no peak has an intensity higher than ~ 3500 counts. Take a Reference Spectrum: Place a cuvette containing the solvent into the USB-ISS-UV/VIS, aligning the triangle at the top of the cuvette with the triangle on the USB-ISS-UV/VIS. Click the Store Reference spectrum icon Take a Dark Spectrum. Completely block the light path going to your sample by deselecting the “shutter open” box on the toolbar. Take the dark spectrum by clicking the Store Dark Spectrum icon. Take an Absorption Spectrum of your Sample: Place a cuvette containing your sample into the USB-ISS-UV/VIS, aligning the triangle at the top of the cuvette with the triangle on the USB-ISS-UV/VIS. Then click the Absorbance Mode icon on the toolbar Set the upper and lower limits of the x- and y-axes: Click the set scale icon and enter appropriate values for the upper and lower limits for absorbance and wavelength. Set the toggle cursor at lambda max: Click the toggle cursor icon and move the green line to lambda max. Note the values for lambda max and the absorbance in the lower left-hand corne