还原型谷胱甘肽对培养乳鼠心肌细胞缺氧-复氧损伤的影响.docVIP

还原型谷胱甘肽对培养乳鼠心肌细胞缺氧/复氧损伤的影响 作者：王进，王一彪，杨海英，赵丽丽，孔春妍 【摘要】 目的 研究还原型谷胱甘肽对乳鼠心肌细胞缺氧/复氧(A/R)损伤的影响。方法 建立心肌细胞A/R损伤模型，随机分为５组：A组，正常对照组；B组，单纯缺氧/复氧(A/R低氧2h，复氧1h)组；C、D、E组为还原型谷胱甘肽处理组，加入还原型谷胱甘肽(GSH)分别使其终浓度为40、80、160 mg/L，后A/R。于复氧后测定各组培养液中乳酸脱氢酶(LDH)变化及细胞内丙二醛(MDA)含量和细胞存活率。结果 与正常组相比，单纯低氧/复氧组LDH漏出量、MDA水平显著升高(Plt;0.01)，细胞存活率显著降低(Plt;0.01)。与缺氧/复氧组比较，各谷胱甘肽处理组上述变化明显减轻(Plt;0.05)。结论 还原型谷胱甘肽对乳鼠心肌细胞A/R损伤具有保护作用，并具有浓度依赖性。 【关键词】 还原型谷胱甘肽 心肌细胞 缺氧 复氧 The effects of reduced glutathione sodium on anoxia/reoxygenation injuries of neonatal rat cardiomyocytes 【Abstract】 Objective To study the effects of reduced glutathione sodium(GSH) on anoxia /reoxygenation injuries of neonatal rat cardiomyocytes.Methods A/R model of myocardial cells of neonatal rats was established. The cells of neonatal rats were randomly divided into five groups: control group(A): without any treatment; A/R group(B):2-hour anoxia followed by 1-hour reoxygenation; GSH conditioned group(C):adding GSH into culture medium till the final concentration of 40mg/L then A/R; GSH conditioned group (D):adding GSH into culture medium till the final concentration of 80mg/L then A/R; GSH conditioned group (E):adding GSH into culture medium till the final concentration of 160mg/L then A/R. The activity of lactate dehydrogenase(LDH)，the contents of cellular melondadehyde(MDA) and the rate of cell viability of each group were evaluated after reoxyenation.Results Compared with group A，the A/R group showed a great increase in levels of LDH，MDA and a decrease in the rate of cell viability(Plt;0.01). Compared with group B，the GSH conditioned groups significantly attenuated these changes(Plt;0.05).Conclusion GSH could be a protective effect on A/R injury of neonatal rat cardiomyocytes in a concentration dependent manner. 【Key words】 reduced glutathione sodium cardiomyocytes anoxia reoxygenation 1 材料与方法 1.1 材料 实验用1～3天的Wistar大鼠乳鼠，山东大学实验动物中心提供，雌雄不拘；DMEM培养基由爱博公司出品；胎牛血清为美国　Gibco公司出品；胰酶为Sigma公司出品；丙二醛（MDA），乳酸脱氢