金丝桃素对慢性高眼压兔视网膜神经节细胞保护作用的组织学观察.docVIP

金丝桃素对慢性高眼压兔视网膜神经节细胞保护作用的组织学观察 【摘要】 　　目的：探索金丝桃素对慢性高眼压兔视网膜神经节细胞的影响。方法：取成年家兔20只，后房内注射α-糜蛋白酶，建立兔慢性高眼压模型。造模成功的兔眼在10d后玻璃体内分别注入金丝桃素0.1mL或生理盐水0.1mL，1次/wk，连续2wk。另用4只正常兔做空白对照。最后光镜电镜观察视网膜神经节细胞的结构变化及视网膜内层平均厚度的变化。结果：生理盐水对照组节细胞数量减少，部分严重变性，染色质边集,线粒体、内质网等细胞器严重肿胀,视网膜内层平均厚度变薄。金丝桃素治疗组节细胞形态结构较生理盐水对照组损伤轻微, 节细胞数量稍有减少,部分变性, 染色质边集，线粒体、内质网肿胀较轻。视网膜内层平均厚度轻度变薄,与生理盐水对照组相比有显著性差异 (P ＜0.01)。 结论：金丝桃素作为一种特异性的蛋白激酶C抑制剂，对慢性高眼压下兔视网膜神经节细胞有保护作用。 【关键词】 金丝桃素　慢性高眼压　视网膜神经节细胞　神经保护　兔 　　The histology observation on the protective effect of hypericin on retinal ganglion cell in chronic ocular hypertension rabbits Abstract AIM: To explore the effect of hypericin on retinal ganglion cell in chronic ocular hypertension rabbits. METHODS: Twenty rabbits were used to build chronic ocular hypertension model by posterior chamber injection of alpha-chymotrypsin. Ten days after successful model creation, hypericin 0.1mL or saline 0.1mL was injected into the vitreous cavity once a week for 2 weeks. Retinal ganglion cell of these eyes and that of four normal rabbit eyes were observed by light microscopy and electron microscopy. The thinckness of inner retina layer was examined.RESULTS: The number of retinal ganglion cell in saline-treated group decreased significantly. There was serious cell degradation with perinuclear condensation of chromatin and swelling organelles, but the change was slight in hypericin-treated group. The difference of the thickness of inner retina layer between the two groups was significant (P＜0.01).CONCLUSION: Intravitreal injection of hypericin as a protein kinase C antagonist can protect the retinal ganglion cell in chronic ocular hypertension rabbits. · KEYWORDS: hypericin; chronic ocular hypertension; retina ganglion cell; nerve protection; rabbits 　　 0引言 青光眼是一组与眼压升高有关的致盲性眼病。它的病理基础是视网膜神经节细胞进行性死亡。目前青光眼的治疗主要是降眼压，但是单纯降眼压并不能阻止视功能的继续下降。随着视神经保护理论的提出和研究，视神经保护治疗越来越受到人们广泛的关注[1]。我们就是基于视神经保护治疗的理论，探讨一种蛋白激酶C（protein kinase C，PKC）抑制剂——金丝桃素对视网膜神经节细胞的保护作用。 1材料和