不同配比黄芪丹参对气虚血瘀证模型大鼠血液流变学及TXB2、6―keto―PGF1α影响的研究.docVIP

不同配比黄芪丹参对气虚血瘀证模型大鼠血液流变学及TXB2、6―keto―PGF1α影响的研究 　　[摘要] 目的 探讨黄芪丹参不同配比对气虚血瘀证大鼠血液流变学及血栓素B2（TXB2）、6-酮前列腺素F1α（6-keto-PGF1α）的影响。 方法 将SD大鼠随机分为正常组、模型组和黄芪丹参不同配比组，每组各10只。黄芪、丹参的总给药量均为原药材15 g/kg，黄芪、丹参比例分别为5∶1、3∶1、1∶1、1∶3、1∶5。采用饥饿、游泳加皮下注射肾上腺素的方法复制气虚血瘀证大鼠模型，各组大鼠给予不同配比的水煎液冻干物，测定血液流变学指标和TXB2、6-keto-PGF1α含量。 结果 气虚血瘀模型组血流变参数和TXB2显著升高，6-keto-PGF1α显著降低，与正常组比较差异有高度统计学意义（P 0.01），模型复制成功。黄芪丹参3∶1、1∶1、1∶3、1∶5配比组可改善血瘀状态，使升高的TXB2水平降低，提升6-keto-PGF1α及6-keto-PGF1α/TXB2比值，差异有高度统计学意义（P 0.01）。 结论 黄芪丹参对大鼠气虚血瘀证确有疗效，其中1∶3配比组效果最佳。 　　[关键词] 丹参黄芪药对；气虚血瘀证；血液流变学；配比研究 　　[中图分类号] R963 [文献标识码] A [文章编号] 1673-7210（2014）08（a）-0013-04 　　Effect of compatibility of Astragalus Membranaceus and Salvia Miltiorrhiza in different proportionon hemorheology and contents of TXB2， 6-keto-PGF1α in blood of Qi deficiency and blood stasis rat model 　　ZHU Lixia1 ZHANG Yingfeng2▲ 　　1.Department of Traditional Chinese Medicine， Zhujiang Hospital of South Medical University， Guangdong Province， Guangzhou 510282， China； 2.School of Chinese Medicine， Guangzhou University of Chinese Medicine， Guangdong Province， Guangzhou 510006， China 　　[Abstract] Objective To explore the influence of the different compatibility proportion of Astragalus Membranaceus and Salvia Miltiorrhiza on Qi deficiency and blood stasis rat model on hemorheology and the content of TXB2 and 6-keto-PGF1α. Methods The SD rats were randomly divided into normal group， model group and five compatibility groups， 10 rats in each group. The Qi deficiency and blood stasis rat model was replicated by restricted diet， forced swimming and norepinephrine subcutaneous injecting. The rats were orally given with different proportion of dry freezing water decoction， the proportions of Astragalus Membranaceus and Salvia Miltiorrhiza were 5∶1， 3∶1， 1∶1， 1∶3， 1∶5 respectively， and the total dosage were all 15 g/kg. The hemorheology and the content of TXB2 and 6-keto-PGF1α were determined. Results The hemorheology parameters and TXB2 were significantly increased and 6-keto-PG