冬虫夏草通过抗氧化及抗衰老减轻糖尿病肾病大鼠肾小管损伤的研究.docVIP

冬虫夏草通过抗氧化及抗衰老减轻糖尿病肾病大鼠肾小管损伤的研究 　　【摘要】 目的：观察冬虫夏草对糖尿病（DM）大鼠肾小管损伤的影响，基于抗氧化及抗衰老探讨冬虫夏草延缓糖尿病肾病进展的作用机制。方法：链脲佐菌素（STZ）诱导糖尿病肾病（DN）大鼠模型，与非DN大鼠一起随机分为四组：对照组、冬虫夏草组、DN组、DN+冬虫夏草组。饲养24周后检测肾脏GPx、SOD、MDA、ROS及Klotho mRNA蛋白表达水平，并检测血肌酐（Scr）、尿素氮（BUN）、24 h尿白蛋白、尿白蛋白肌酐比（UACR）、肌酐清除率（Ccr）。结果：DN组大鼠肾功能明显降低，肾脏氧化应激水平增加，肾小管Klotho mRNA及蛋白表达下降。经冬虫夏草治疗后肾功能明显改善；ROS和MDA水平降低，SOD及GPx水平增加；肾小管Klotho表达上调。结论：冬虫夏草可延缓DN进展，其机制可能与其在肾小管间质发挥的抗氧化及抗衰老作用有关。 　　【关键词】 冬虫夏草； 糖尿病肾病； 氧化应激； Klotho 　　【Abstract】 Objective：To observe the effect of cordyceps on tubular injury in diabetic rats， and explore whether the reno-protective action has a close relationship with anti-oxidation and anti-aging.Method：The rats were induced into diabetes mellitus （DN） by intraperitoneal injection of streptozotocin（STZ）.They were randomly divided into four groups：the namely control group，the cordyceps group，the DN group，the DN+cordyceps group.At the 24th weeks，the protein expression of Klotho mRNA level in tubule and levels of GPx，SOD，MDA，ROS in renal cortex were studied.Scr，BUN，urinary albumin，UACR and Ccr were also investigated.Result：Compared with the control group，renal function was decreased obviously in DN group.The Klotho protein and mRNA levels were also decreased，but renal oxidative stress was increased.However，cordyceps treatment could significantly improve the renal function，increase the SOD and GPx levels，the protein and mRNA expression of Klotho，but lower the MDA and ROS levels.Conclusion：Cordyceps sinensis can delay the progress of DN，its mechanism may be associated with renal tubular interstitial play a role of the antioxidant and anti-aging. 　　【Key words】 Cordyceps； Diabetic nephropathy； Oxidative stress； Klotho 　　First-author’s address：Institute Nephropathy，Affiliated Hospital of Guangdong Medical College，Zhanjiang 524000，China 　　doi：10.3969/j.issn.1674-4985.2014.22.005 　　大量研究表明，DN其实是典型的衰老相关性疾病，肾脏多种固有细胞的衰老和功能减退（对抗损伤的应激能力减弱）是DN各种病理损害加速进展的重要原因[1-2]。Klotho基因是近年来新发现的抗衰老基因，其在肾小管间质高表达，提示它在肾脏疾病的发生和发展过程中很可能发挥着重要的调控作用。冬虫夏草在肾