利拉鲁肽对高脂饮食喂养大鼠血清炎症因子的影响及对大动脉的保护作用.docVIP

利拉鲁肽对高脂饮食喂养大鼠血清炎症因子的影响及对大动脉的保护作用 　　摘 要：目的：观察利拉鲁肽对高脂饮食喂养大鼠血清炎症因子的影响，探讨利拉鲁肽对大动脉保护作用的机理。方法：60只清洁级SD雄性大鼠随机分为正常对照组、高脂饮食组、低剂量利拉鲁肽干预组、高剂量利拉鲁肽干预组，分别予以正常饮食、促动脉硬化高脂饮食、以及不同剂量利拉鲁肽处理12周后。酶联免疫ELISA法检测处理后血清hs-CRP、ICAM-1、VCAM-1水平，彩超及HE染色检测大鼠腹主动脉病变情况。结果：与对照组相比，促动脉硬化高脂饮食组大鼠体重增加，血清hs-CRP、ICAM-1、VCAM-1升高，腹主动脉内中膜厚度增厚；利拉鲁肽干预组大鼠体重增加不明显，血清hs-CRP、ICAM-1、VCAM-1水平及腹主动脉内中膜增厚情况有所减轻，以高剂量利拉鲁肽干预组改善更明显（P0.05）。结论：1、利拉鲁肽对高脂饮食喂养大鼠的腹主动脉内中膜厚度有保护作用，该作用呈剂量依赖性。2、利拉鲁肽对高脂饮食诱导大血管损伤的大鼠血管的保护作用可能与改善血清炎症因子水平有关。 　　关键词：利拉鲁肽、血清炎症因子、高脂饮食、腹主动脉内中膜厚度。 　　Abstract：Objective： To observe the effects of liraglutide on the aortic intima-media thickness in rats fed with promoting atherosclerosis high fat diet， investigate its possible mechanism.Methods： 60 clean SD male rats were randomly divided into 4 groups：Normal diet group， promoting atherosclerosis high fat diet group， high fat diet +low-dose liraglutide group， high fat diet+ high-dose liraglutide group，correspondingly given ordinary diet or promoting atherosclerosis high fat diet for 12 weeks. The body weight，serum hs-CRP、ICAM-1、VCAM-1 were measured at the end of experiment. The intima mediathickness of abdominal aortic was detected by ultrasound. Kesults： Compared with the control group， the body weight， serum hs-CRP、ICAM-1、VCAM-1 level of rats on high fat diet was significantly increased. The abdominal aortic intima-media thickness was increased in rats fed with high fat diet. Liraglutide intervention ameliorated the abdominal aortic intima-media thickness in a dose dependent manner. The serum hs-CRP、ICAM-1、VCAM-1 level were decreased in all Liraglutide intervention groups.Conclusions： Liraglutide ameliorated the abdominal aortic intima-media thickness in rats fed with promoting atherosclerosis diet in a dose dependent manner. The protective effect of Liraglutide on macrovascular lesions may relate to an decrease of the serum inflammatory factors level. 　　Keywords： Liraglutide； serum inflammatory factors；High-fat diet；Macrovascular lesions 　　利拉鲁肽（Liraglutide）