生防菌NH-BS-2000对西瓜枯萎病的防治效果及其促生作用.doc

生防菌NH-BS-2000（Bacillus subtilis）对西瓜枯萎病的防治效果及其促长作用 朱育菁，葛慈斌，蓝江林，林抗美，肖荣凤* （福建省农科院生物技术研究所，福建 福州 350003） 摘 要：为了实现对西瓜枯萎病进行生物防治，研制出能替代化学农药的生物农药，本研究进行枯草芽孢杆菌NH-BS-2000对西瓜枯萎病的防治效果及促长作用的田间小区试验。试验结果表明：生防菌NH-BS-2000对西瓜苗期枯萎病的防治效果可达61.62%，高于化学药剂三环唑和维线克41.65%和37.55%，表现在能促进植株根部的生长。在处理30 d后，生防菌处理组对西瓜枯萎病的防治效果达83.14%，显著高于三环唑处理组和维线克的18.09%和25.53%，表明生防菌比化学农药具有更长的持效期，同时还能促进西瓜植株的株高、叶片数、着花数、座果数、叶长和叶宽等的生长。说明NH-BS-2000菌株具备作为枯萎病生防菌的良好优势，有望开发出瓜类枯萎病的生防菌剂。研究还发现生防菌NH-BS-2000的施用影响了土壤根际的微生物群落，显著提高了放线菌的含量。 关键词：生防菌NH-BS-2000；枯草芽孢杆菌；西瓜枯萎病；防治效果；促长作用 中图分类号： 文献标识码： 文章编号： Control Efficacy of Biocontrol Strain NH-BS-2000 (Bacillus subtilis) against Wilt Disease of Watermelon and its Promoting Effect on the Growth ZHU Yu-jing, GE Ci-bin, LAN Jianglin, LIN Kanmei, XIAO Rong-feng, LIU Bo (Institute of Biotechnology, Fujian Academy of Agricultural Sciences，Fuzhou 350003，China) Abstract：The field experiment on the control efficacy of biocontrol strain NH-BS-2000 (Bacillus subtilis) against wilt disease of watermelon and its promotiong effect on the growth was conducted at Fuzhou, Fujian, China. The result showed that the control efficacy of NH-BS-2000 against the wilt disease of watermelon seedlings was 61.62%, higher than 41.65 nad 37.55% of chemical fungicide Tricyclazole and Weixianke, respectively. At 30 days after treatments, NH-BS-2000 had a control efficacy of 83.14%, which was significantly greater than 18.09% of Tricyclazole and 25.53% of Weixianke, and it also promted the development of plant height, leaf number, flower number, fruit number, leaf height and leaf width. The results indicated that NH-BS-2000 could be a good biocontrol strain for the development of a bio-fungicide against wilt disease of melon. Besides, the usage of NH-BS-2000 increased the number of actinomycete at the rhizospheric soils of watermelon seedlings. Keywords：Biocontrol strain NH-BS-2000; Bacillus subtilis; wilt disease of watermelon; control efficacy, promoting effect on growth 西瓜枯萎病是由尖孢镰刀菌西瓜专化型（Fusa