PowerPoint Presentation Gas ChromatographyPowerPoint演示文稿气相色谱法.pptVIP

The peak height is proportional to the amount of material eluting from the column at any given time, The area under the peak is a measure of the total amount of material that has eluted from the column. Electronic integrators are used for area measurement in commercial GCs. We will be using ALGEBRA. ? Determination of the Amount of Sample Components Present wb h Area = 1/2 wb h The Gaussian curve can be approximated as triangular in shape, to simplify area measurement. NOTE: the height is measured to the top of the tangents, which is above the actual curve peak. Blue Flame Blue Flame Green Flame retention time peak width If voltage data is very noisy, resulting in poor peak shape, some peak parameters may be estimated from visual observations, however areas cannot be calculated. So have the TA verify your data before you take apart your GC. onset of green end of green Collect voltage vs time data and also note visual onset and disappearance of green flame color. Experiments 1. Test run of CH2Cl2 without sensor check for visible color, reasonable width and retention time on column. 2. Run of Pure Compounds: (1 good run of each) CH2Cl2 (Dichloromethane aka Methylene Chloride) CHCl3 (Chloroform) 3. Mixture: CH2Cl2:CHCl3 (2:3 mix) Data and Calculations A. Graphs (3) from computer 1. Elution data for pure CH2Cl2 2. Elution data for pure CHCl3 3. Elution data for mixture B. Calculations (handwritten, for each graph) 1. Peak Area = 1/2 (W x H) 2. Number of theoretical plates, N = 16 (TR / Wb)2 (There is a separate handout available with this information.) Checkout 1-pipet (5 ml graduated, for GC column) 1-flow regulator (buret valve) 1-clothespin 1-pair forceps 1-1cc syringe 1-flame shield (black construction paper cylinder) 1-sensor/stopper 1-coil adjusting tool 1-pc Cu wire (discard after use) In Hoods: Tide (Replace lid to prevent moisture absorption) teaspoons plastic funnels fiberfill (looks like cot