Reaction discovery enabled by DNAtemplated synthesis and in发现DNA模板合成反应的启用和.pptVIP

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2017-03-08 发布于上海
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Reaction discovery enabled by DNAtemplated synthesis and in发现DNA模板合成反应的启用和.ppt

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Reaction discovery enabled by DNAtemplated synthesis and in发现DNA模板合成反应的启用和

BioIntelligence Lab Reaction discovery enabled by DNA-templated synthesis andin vitro selection Matthew W. Kanan, Mary M. Rozenman, Kaori Sakurai, Thomas M. Snyder David R. Liu What they did? A reaction discovery approach that uses DNA-templated organic synthesis and in vitro selection to simultaneously evaluate many combination of different substrates for bond-forming reactions in a single solution How? – Strand Preparation Strand Preparation in detail Recent developments in DNA-templated organic synthesis indicate that DNA annealing can organize many substrates in a single solution into DNA sequence-programmed pairs Two pools of DNA-linked substrates, with n substrates in pool A and m substrates in pool B Each substrate in pool A is covalently linked to the 5` end of a set of DNA oligonucleotides containing one ‘coding region’ (uniquely identifying that substrate) and one of m different ‘annealing regions’ Each of the m substrates in pool B is attached to the 3` end of an oligonucleotide containing a coding region that uniquely identifies the substrate and complements one of the m annealing regions in pool A How? – Reaction Separation Bond forming Reaction in detail Role of Watson-Crick base pairing When pools A and B are combined in a single aqueous solution, Watson–Crick base pairing organizes the mixture into n x m discrete pairs of substrates attached to complementary sequences Only substrates linked to complementary oligonucleotides experience effective molarities in the millimolar range Possibility of interference by the DNA structure Minimized by using long and flexible substrate–DNA linkers Separation in detail Separation Avidin-Biotin in detail Avidin/streptavidin-biotin systems are particularly useful as a bridging or sandwich system in association with antigen-antibody interactions Biotin and Avidin Biotin: a small organic molecule found in every cell Avidin: a much larger protein that binds biotin with a very high affinity When these