Zebrafish Development conj542斑马鱼 conj542.biostr.washington.pptVIP

Early Development Movies dorsal Early Development Movies Two major Questions How are the germ layers established? What determines polarity? Signaling from the YSL Nodal mutant wild-type sqt;cyc -/- No endoderm No head trunk mesoderm Normally ~1550 cells internalize; in MZoep ~60 internalize Germ layer patterning YSL signal Sqt and Cyc Endoderm Head and Trunk Mesoderm Tail mesoderm Dorsal-ventral patterning Shield (Organizer) Bozozok The Organizer Shield (Organizer) Bmp promotes ventral fates Organizer signals are BMP binding factors: Noggin, Chordin Bmp signals promote ventral fates (skin, ventral mesoderm) and oppose dorsal fates (neural, dorsal mesoderm) Mutants in the dorsal-ventral pathway Snailhouse (snh) = bmp7 mutant Chordino (din) = chordin mutant Bmp regulates cell movement High Bmp Low Bmp Summary Gastrulation It is not birth, marriage or death, but gastrulation, which is truly the most important event in your life Lewis Wolpert 17 hours in the life of a zebrafish embryo Blastula Stages 1-cell 2-cell 4-cell 8-cell 32-cell 512-cell 1000-cell Transcription begins sphere dome 30% epiboly 4 2/3 hrs Gastrula Stages 50% germ ring shield 75% 90% bud 10 hr Somitogenesis 3-somite 6-somite 18-somite 26-somite 22 hr Zebrafish development Early development ? Three germ layers Side view top view Skin nervous system Muscle cardiovascular Gut and liver Animal pole Vegetal pole Gastrulation simplified Involution Mesoderm endoderm inside Ectoderm outside Dorsal-Ventral Side view top view dorsal dorsal ventral ventral Involuting cell movements during gastrulation Convergence-Extension convergence extension Convergence-Extension Convergence-extension Cell fates Dorsal view Cross section dorsal dorsal Convergence-extension Convergence-extension Slow dorsal migration Convergence Extension Convergence-extension cell movements Slow dorsal migration Fast dorsal migration Convergence Extension Convergence-extension cell movements Slow dorsal migrati