下一代测序在表观遗传学中的重要应用及进展_沈圣解剖.pdf

Hereditas (Beijing) 2014 3 , 36(3): 256 275 ,, , 79905 (Next generation sequencing, NGS) , NGS , NGS , (Whole genome bisulfite sequencing, WGBS) (Reduced representation bisulfite sequencing, RRBS) DNA (Methylated DNA immunoprecipitationsequencing, MeDIP-seq) (Chromatin immunoprecipitation-sequencing, ChIP-seq) Tet (Tet-assisted bisulfite sequencing, TAB-seq) (Chromosome conformation capture se- quencing, 3C-seq) Dnase -seq/MNase-seq/FAIRE-seq RNA (RNA sequencing, RNA-seq) , , NGS , NGS NGS , ; ; DNA ; ; The application of next generation sequencing on epigenetic study Sheng Shen, Yanchun Qu, Jun Zhang COE for Neuroscience, Texas Tech University Health Sciences Center, El Paso, Texas 79905, USA Abstract: The application of next generation sequencing (NGS) technique has a great impact on epigenetic studies. Coupled with NGS, a number of sequencing-based methodologies have been developed and applied in epigenetic studies, such as Whole Genome Bisulfite Sequencing (WGBS), Reduced Representation Bisulfite Sequencing (RRBS), Methylated DNA Immunoprecipitation Sequencing (MeDIP-seq), Chromatin Immunoprecipitation-Sequencing (ChIP-seq), TAB-seq (Tet-assisted Bisulfite Sequencing), Chromosome Conformation Capture Sequencing (3C-seq) and various of 3C-seq derivatives, DNase -seq/MNase-seq/FAIRE-seqand RNA Sequencing (RNA-seq). These new techniques were used to identify DNA methylation patterns and a broad range of protein/nucleic acid interactions, and to analyze chromatin conformation.With these new technologies, researchers have gained a broader view and better tools to investigate the distributions and dynamic changes of epigenetic markers affected by both internal and external factors. The principles and characteristics of major applications of NGS technologies on epigenetics were summarized; and the recent advances and the future direc- : 2013?10?16; : : 2013?12?07 / Coldwell :, , E-mail: saint_ss@163.com :, , , E-mail: jun.zhang2000@ DOI: 10.3724/SP.J.1005.2014.0256 :