人血管内皮细胞与丝素材料粘附初期细胞的生化变化.pdfVIP

人血管内皮细胞与丝素材料粘附初期细胞的生化变化* 1 1 1 2 秦博 ，方凯正 ，赵东旭 ，张志谦 （1，北京理工大学生命科学与技术学院，北京 100081 ；2 ，北京市肿瘤研究所，北京 100035 ） [摘要] 目的：研究人血管内皮细胞与丝素材料粘附初期细胞微管骨架和整合素β3的变化，以认识材料内 皮细胞化的过程中内皮细胞与丝素材料之间的粘附机理。方法：以未铺丝素膜的聚苯乙烯板为对照，采用 细胞间接免疫荧光的方法观察在材料上生长不同时间的细胞微管形态变化，并采用流式细胞术检测不同时 间点细胞表面整合素β3 的量变。结果：微管骨架系统会随着生长时间的推移而逐渐在其所粘附的材料表面 进行铺展，而整合素β3在粘附初始阶段和完全粘附阶段的表达量大致持平，而在粘附加深阶段，整合素β3 大量表达。 [关键词]人血管内皮细胞；丝素蛋白；粘附初期；微管骨架；整合素β3 The Human Vascular Endothelial Cell’s Biochemical Changes in Process of Adherent to Fibroin Film 1 1 1 2 Qin BO ，Fang Kaizheng ，Zhao Dongxu ，Zhang Zhiqian （1，School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081 ；2 ，Beijing Insttute of Cancer, Beijing 100035 ） Abstract: Objective: To study human vascular endothelial cell’s changes of microtubulin skeleton and expression of integrin β3 in process of adherent to fibroin film so as to adhesive mechanism during the endothelialization process of materials. Method: With cell culture plate as the control material, the cell indirect immunofluorescent technology was used to observe the changes of microtubulin skeleton and flow cytometry method was used to detect the expression of integrin β3 of the cell growing on materials in different time stages. Result: The cell’s microtubulin skeleton spread gradually and the amount of expression of the integrin β3 is roughly the same in the initial adherent stage and the stage of adhesion completely, but the amount of integrin was expressed largely in the adherent strengthening stage. Key Words : HUVEC; Silk fibroin film; Initial adherent stage; Cell microtubule skeleton; Integrin β3 * 973 计划资助课题（2005CB623906）