基于盐碱性环境下产腈水合酶的微生物筛选及其发酵水合反应的初步研究.pdfVIP

J030 基于盐碱性环境下产腈水合酶的微生物筛选及其发酵 水合反应的初步研究1 2 李 春 ，张根林，李东峰 （石河子大学生物化工实验室，新疆石河子 832003 ） 摘 要 利用含有一定比例丙烯腈和丙烯酰胺的特制培养基从新疆盐碱环境下筛选和分离产腈 水合酶微生物，获得了具有在较高盐浓度和pH 偏碱性条件下生长良好、菌苔为光滑、橙红、湿 润的菌株。研究其细胞培养特性，通过对菌株的极端定向培育和产酶过程的研究，采用了葡萄糖 －诱导剂耦合补加工艺进行催化剂的制备，并进行水合反应的初步研究。结果表明，pH ＝7.2 和 葡萄糖浓度维持在 3～5gl－1 时产生的腈水合酶酶活可达 5000Uml－1,水合后丙烯酰胺最终浓度可 －1 达527gl 。 关键词 腈水合酶 丙烯酰胺 盐碱环境 菌株筛选 SCREENING A MICROORGANISM OF PRODUCING NITRILE HYDRATASE UNDER THE SALINE AND ALKALINE ENVIRONMENT AND PRELIMINARY STUDY ON FERMENTING AND HYDRATING LI Chun, ZhANG Genlin, LI Dongfeng （Lab. Of Biochemical Engineering, Shihezi University. Shihezi 832003, Xinjiang ） Abstract A nitrile hydratase(NHase) producing microorganism was screened and separated under the saline and alkaline environment from Xinjiang by ordering-built culture of containing certain proportion acrylonitrile and acrylamide .Bacterial strain that grow well in high concentration of salt and pH towards alkaline was attained , which bacterial lawn is smooth, orange red and moist. A novel technics of glucose—revulsant coupling fed was adopted to prepare the NHase by studying the cell growth, extreme directional cultivation of strain and the process of producing NHase. The result showed , the NHase activity was increased to 5000 Uml －1 －1 under pH =7.2 and glucose concentration at maintaining 3-5 gl . The final concertration of －1 acrylamide reached 527 gl after the hydration. 1基金项目：新疆自治区重点科技攻关项目资助（No. XJKJGG004 ）。 2通讯联系人： 李春 博士、教授、博士生导师，email ：lichun@tsinghua.org.cn Keywords NHase ,,acrylamide , the saline and alkaline environment, s